2×Hieff™ Ultra-Rapid II HotStart PCR Master Mix contains heat-stabilized Taq DNA Polymerase modified with antibodies, adds strong elongation factor and optimized buffer system, and has super high amplification efficiency. It is very suitable for PCR amplification of most colonies such as E. coli, Agrobacterium, and yeast. The extension speed of amplification of complex templates within 3 kb can reach 1 sec/kb, 3-6 kb to 3 sec/kb, 6-10 kb to 5 sec/kb and 10 kb to 10 sec/kb; the amplification speed of simple templates such as plasmid within 6 kb can reach 1 sec/kb, which can greatly save PCR reaction time. Meanwhile, Mix contains dNTPs, Mg²⁺, and it can be amplified only with adding primers and template. In addition, Mix contains red tracer dye, which can be used by electrophoresis directly after the end of the reaction. The protective agent added to the system enables the product to maintain a stable activity after repeated freezing and thawing. The 3′ end of the PCR product bands A and can be easily cloned into the T vector.

Features

Faster – Bacterial plasmids up to 3 kb can be processed in as little as 1 second per kb.

More Versatile – Efficiently handles GC content up to 70% and fragment lengths up to 10 kb.

More Reliable – Validated on hundreds of fragments across over ten species.

Higher Efficiency – High specificity with detection rates up to 100%.

More Stable – Maintains stability after 100 freeze–thaw cycles, with a shelf life of up to two years.

Applications

Crispr PCR

Colony Direct PCR

Genotyping

Specifications

Figures

1. Crispr Genotyping

Cited from: Viruses 2024, 16(9), 1382;

2. Nest PCR

Cited from: Front. Microbiol. 15, 2024

3. Colony Direct PCR

Fig 1. Fast Colony Direct PCR with E. coli and Agrobacterium samples using 4 different speed of 1: 1s/kb, 2: 3s/kb, 3: 5s/kb and 4: 10s/kb. M: 10,000 DNA Marker (10505ES).

4. Long PCR with Genomic DNA

Fig. 2. Fast amplification of 1-10 kb mouse gDNA shows high yield and a high speed at 10 sec/kb. M: 10,000 DNA Marker (10505ES).

5. High Stability