وصف
YeaRed is a unique oily macromolecule that can't penetrate cell membranes into cells. It is not easy to be volatile and sublimation and the human body will not inhale. The Ames test results also showed that YeaRed is completely mutagenic at gel staining concentration and is a safe and non-toxic nucleic acid dye. YeaRed has the same spectral characteristics as EB, and there is no need to change the filter and observation device (ordinary UV gel transilluminator), and the UV light excitation detection at 300 nm is sufficient. YeaRed is suitable for dsDNA, ssDNA, and RNA staining in agarose and polyacrylamide gel electrophoresis, and can be stained by glue dyeing method or bubble dyeing method, which is very convenient and flexible to use.
Features
- High Sensitivity: Comparable to EB sensitivity
- Strong Stability: Microwaveable, stored at room temperature
- Wide applicability: Applicable for DNA or RNA staining
- Safe and Non-Toxic: Ames test shows no caccinogenic toxicity
- Easy to Dispose: Waste Yeared can be directly disposed of without environmental pollution
- Perfect Replacement: With the same spectral properties as EB
Applications
- Sensitive fluorescent dyes for nucleic acid detection
Specifications
| Detection of positioning | Test in gel |
| Detection method | Fluorescence |
| Product Type Specs | Nucleic acid gel dye |
| Condition of carriage | Room temperature |
| Target molecule | DNA, RNA |
Components
| Components No. | Name | 10202ES76 |
| 10202 | YeaRed™ Nucleic Acid Gel Stain (10,000× in Water) | 500 μL |
Shipping and Storage
The product is shipped at room temperature and avoids light. It can be stored at room temperature for 5 years.
FAQ
Q: Are there any differences between YeaRed in DMSO solution and in water solution?
A: The 10202ES water-soluble version is a completely new improvement. Since DMSO can be absorbed by the skin, it is recommended that the dye be dissolved in water to avoid potential hazards associated with handling DMSO. In the agarose gel electrophoresis experiment, the two solvents performed equally well. For other applications, customers can choose based on their needs.
Q: Can the YeaRed nucleic acid dye used in the perming process be reused?
A: It can be reused about 3 times.
Q: What types of samples are suitable for YeaRed nucleic acid dye?
A: Is it suitable for electrophoretic staining of various-sized fragments of dsDNA, ssDNA and RNA?
Q: What are the differences between the two dyes, YeaRed (10202) and YeaGreen (10204)?
A: The excitation wavelength of YeaRed is 300 nm, and it needs to be observed under an ultraviolet imaging instrument; the excitation wavelength of YeaGreen is 471 nm, and it needs to be observed under a laser imaging instrument or a visible light imaging instrument.
Q: What is the binding mechanism of YeaRed?
A: Through the combination of electrostatic forces and charge interactions.
Q: Why do the bands sometimes show a trailing or twisting phenomenon after staining?
A: YeaRed is an oily macromolecule. YeaRed forms a strong electrostatic bond with nucleic acids. When the amount of nucleic acid sample is high, it may affect the migration rate of nucleic acid molecules, causing tailing or band deformation. In such cases, it is recommended to reduce the sample loading amount and dilute the marker.
Q: Which downstream experiments are suitable for YeaRed nucleic acid dye?
A: Compatible with the recycling of gel, as well as downstream experiments such as cloning and sequencing.
Q: Can it be stored at -20℃? What should be done if it is accidentally placed at -20℃?
A; It cannot be stored at -20℃. Doing so may cause the dyes to freeze and form precipitates. If it is accidentally placed at -20℃ for a short period of time, you can try to thaw it, then heat it at around 35℃ for 1-2 minutes, and use it after that. Do not repeatedly freeze and thaw it afterwards.
Q: What is the emission wavelength of this dye?
A: The emission wavelength is 590nm.
Documents:
Safety Data Sheet
Payment & Security
تتم معالجة معلومات الدفع الخاصة بك بشكل آمن. لا نقوم بتخزين تفاصيل بطاقة الائتمان ولا يمكننا الوصول إلى معلومات بطاقة الائتمان الخاصة بك.
You may also like
Inquiry
FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.