Hieff Canace™ Long PCR Master Mix (With Dye) is a ready-to-use 2× pre-mixed solution containing Hieff Canace™ High-Fidelity DNA Polymerase, dNTPs, and an optimized buffer system, which contains pre-added electrophoresis indicators. The pre-mix contains pre-added electrophoresis indicator, PCR products can be directly electrophoresed, the amplification products are flat ends. 2× Hieff Canace™ Long PCR Master Mix (With Dye) has the advantages of quick and easy, high sensitivity, high specificity, good stability, etc., the reaction system can be added with only the primers and templates. In addition, the product also contains a specific protective agent, so that the premix can still maintain stable activity after repeated freezing and thawing.
Ultra-high fidelity: Low mismatch rate in mutation-prone high GC gene;
Fast speed: The speed is as fast as 5 sec/kb;
Wide applicability: It can be used to amplify genes with 20-80% GC content, and tolerate 20% blood and mouse lysates;
Good stability: Stored at 37°C for 7 days without affecting performance;
Easy use: Just adding primers and templates for amplification, with blue loading dye for direct electrophoresis.
Specifications
|
Cat NO. |
10166ES01 / 10166ES03 / 10166ES08 / 10166ES25 / 10166ES30 |
|
Size |
250 μL / 1 mL / 5×1 mL / 25×1 mL / 25 mL |
Components
|
Name |
10166ES01 |
10166ES03 |
10166ES08 |
10166ES25 |
10166ES30 |
|
Hieff Canace™ Veritas Long PCR Master Mix (With Dye) |
250 μL |
1 mL |
5×1 mL |
25×1 mL |
25 mL |
Figures
Figure1. High sensitivity of Hieff Canace™ High-Fidelity Long PCR Master Mix.
Figure 2. Long PCR from cDNA by Hieff Canace™ High-Fidelity Long PCR Master Mix.
Notes
- This product is for research use only.
- Please operate with lab coats and disposable gloves，for your safety.
Storage
FAQ
(2) If the RNA sample is small or if you need to obtain a very long target fragment, it is recommended not to perform digestion during reverse transcription. The longer the target fragment, the more RNA needs to be input, and accordingly, more cDNA will be required.
(2) For animal genomes, after product performance testing, the minimum detectable amount is 100pg of animal genome.
(3) For plasmids, since they are relatively pure, after product performance testing, the minimum detectable amount is 1pg of plasmid.
(1)The thawing requirements for 10166ES stipulate that it should be thawed slowly on ice, and it cannot be thawed at room temperature or through heating.
(2) When performing the amplification process, it is recommended to set the temperature at 68℃ and the amplification speed at 10 seconds per kilobase.
(3) The longer the target fragment to be amplified is, the more template should be used.
(4) In the PCR system, the concentration of primers is too low or the number of cycles is insufficient: appropriately increase the amount of primers, or increase the number of amplification cycles;
Citations
Applications
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FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.