Synonyms

IFN-R-2;IFNAR2;IFNARB;IFNABR;IFN-alpha; IFN-alpha/beta R2;IFNbR2;IFN-R;β receptor 2;β receptor10;

Species

Cynomolgus

Expression Sequence

Ile27-Lys243

Accession

EHH53981.1

Tag

C-His

Expression System

HEK293

Molecular Weight

The protein has a predicted MW of 25.93 kDa. Due to glycosylation, the protein migrates to 43-60 kDa based on Bis-Tris PAGE result.

Purity

> 95% as determined by SDS-PAGE and SEC-HPLC

Endotoxin

Less than 0.01 EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological activity

SPR Data: Cynomolgus IFN alpha/beta R2, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 70.28 nM as determined in SPR assay .

Reconstitution Method

Dissolve the lyophilized protein in distilled water. For vials containing 25 μg or 100 μg, we recommend reconstituting to a concentration of 0.5 mg/mL. For larger quantities (500 μg or 1 mg), a concentration of 1 mg/mL is suggested. During reconstitution, avoid vigorous vortexing or shaking. Aliquot the reconstituted solution as needed and store at -80°C. Avoid repeated freeze-thaw cycles.