Sự miêu tả
Flt-3 Ligand (Fms-related tyrosine kinase 3 ligand, FLT3L) is a key cytokine supporting the proliferation, survival, and maintenance of early hematopoietic progenitor cells and dendritic cells. In HSC expansion systems, FLT3L promotes the self-renewal and output of multipotent progenitor cells. In iPSC-to-HSC differentiation protocols, it acts during the early hematopoietic specification stage, supporting the emergence of hemogenic endothelial cells and the subsequent formation of hematopoietic progenitor cells.
Product Properties
|
Item |
Specification |
|
Synonyms |
FL, FLG3L, Flt3L, SL Cytokine |
|
Species |
Human |
|
Expressed Sequence |
Thr27-Pro185 |
|
Accession |
P49771 |
|
Tag |
No Tag |
|
Expression System |
CHO |
|
Molecular Weight |
The protein has a calculated MW of 18.0 kDa. The protein migrates as 24 kDa, 27 kDa±3 kDa when calibrated against the Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
|
Purity |
> 95% as determined by SDS-PAGE. |
|
Endotoxin |
< 0.01 EU/μg protein as determined by LAL method. |
|
Formulation |
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with trehalose as protectant. |
|
Appearance |
Lyophilized Powder |
|
Biological Activity |
The ED50 as determined by the dose-dependent stimulation of the proliferation of human OCI-AML5 cells is ≤ 2.0 ng/mL. |
|
Reconstitution Method |
It is recommended to reconstitute the protein in sterile PBS (pH 7.4) to a concentration of 0.1-1.0 mg/mL. Avoid repeated freeze-thaw cycles whenever possible. |
Features
- High purity: >95%, preventing immune reactions caused by residual host cell protein (HCP) or DNA.
- Ultra-low endotoxin: <10 EU/mg, minimizing the risk of fever or inflammation.
- Batch-to-batch consistency: ensuring stability and reproducibility in cell culture.
- Regulatory compliance: meeting regulatory requirements for cell therapy products and supporting IND submissions.
- Animal-origin-free: free of animal viruses, pathogenic agents, and adventitious agent contamination, ensuring high safety.
Applications
SDS-PAGE
Figure 1. The purity is greater than 95% as determined by SDS-PAGE under reduced condition.
Bioactivity
Figure 2. The ED50 as determined by a cell proliferation assay using human AML5 cells is less than 2.0 ng/mL.
Storage
Store at -25 to -15°C, valid for 3 years. After reconstitution, store at -85 to -65°C, valid for 3 months.
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