Sự miêu tả
HieffTM Plant DNA Kit adopts HieffTM DNA Column P1 and a new solution system, which is suitable for fast and easy extraction of genomic DNA from plant tissues (including plants containing phenols, polysaccharides and enzyme inhibitors), cells and fungal samples. In the extraction process, toxic organic substances such as phenol and chloroform are not needed to be extracted, and time-consuming isopropanol or ethanol precipitation is not needed. The operation is simple and can complete DNA extraction and purification of plant samples (100 mg fresh plant tissue or 20 mg dried plant samples) within 30 min. The HieffTM DNA Column in the kit can specifically adsorb nucleic acids, and does not adsorb protein, polysaccharide and other non-nucleic acid substances, so it is efficient, rapid and convenient. The extracted plasmid DNA has high purity and can be directly used in subsequent experiments such as PCR, enzyme digestion and hybridization.
Feature
- Wide Sample Compatibility: Efficiently extracts DNA from a variety of sources, including common plants, challenging plant tissues rich in polysaccharides and polyphenols, cells, and fungi.
- High-Purity DNA: Delivers DNA free of protein and RNA contamination—ideal for PCR, restriction digestion, and NGS library preparation.
- Safe & Environmentally Friendly: No hazardous organic solvents such as phenol or chloroform required.
- Fast & User-Friendly: Streamlined protocol enables DNA extraction in just 30 minutes.
Components
|
Components No. |
Name |
18800ES08 (5 T) |
18800ES50 (50 T) |
18800ES70 (200 T) |
|
|
Part I |
18800-A |
RNase A (10 mg/mL) |
25 µL |
250 µL |
1 mL |
|
Part II |
18800-B |
HieffTM DNA Adsorption Column P1 |
5 |
50 |
200 |
|
18800-C |
2 mL Collection Tube P1 |
5 |
50 |
200 |
|
|
18800-D |
Lysis Buffer（LB Buffer P1） |
2.5 mL |
25 mL |
100 mL |
|
|
18800-E |
Binding Buffer（BD Buffer P1） |
1 mL |
10 mL |
40 mL |
|
|
18800-F |
Deproteinization Solution PL(PL Buffer P1*) |
1.5 mL |
15 mL |
50 mL |
|
|
18800-G |
Wash Buffer* |
1.5 mL |
13 mL |
50 mL |
|
|
18800-H |
Elution Buffer |
1 mL |
10 mL |
20 mL |
|
Shipping and Storage
Part I components are transported in ice bags and stored at -25~-15℃;
Part II components are transported at room temperature and stored at room temperature.
The product is valid for 2 years.
Application
Plant DNA extraction; Genomic DNA isolation (from plants); Plant DNA isolation
Figures
1. High Yields
Figure 1. Comparison of Plasmid DNA Yields from Arabidopsis thaliana and Populus using Kit 18800(Y1/Y2) and Supplier T*(T1/T2). After elution with 200 µL ddH2O, 5 µL was analyzed by gel electrophoresis. Kit 18800 showed higher DNA yield.
Documents:
Safety Data Sheet
Manuals
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