The Hieff NGS™ MagSP rRNA Removal Kit (Bacteria, G⁻ and G⁺) with Purification Beads uses streptavidin-labeled magnetic beads to deplete ribosomal RNA from bacterial (Gram-negative and Gram-positive) total RNA, thereby enriching messenger RNA (mRNA) and other non-coding RNAs. The kit delivers efficient rRNA removal from both intact and partially degraded total RNA. The rRNA-depleted RNA is suitable for downstream applications including high-throughput sequencing of mRNA and non-coding RNA—which markedly increases the proportion of informative reads—as well as cDNA synthesis and other downstream uses.

Features

• Pan-bacterial ribosomal RNA removal kit (universal across bacterial species).

• Does not include archaeal depletion.

• Magnetic-bead workflow with broad species probe coverage.

Applications

• Microbial transcriptomics.

• Meta-transcriptomics.

Storage

Box I (Probe Mix): store at -25 to -15°C. Box II: store at 2 to 8°C.