Hieff NGS™ OnePot Pro DNA Library Prep Kit V4 is is an advanced enzymatic DNA library preparation kit designed for compatibility with both Illumina™ and MGI™ next-generation sequencing (NGS) platforms. By utilizing high-efficiency enzymatic fragmentation, this kit eliminates the need for traditional mechanical shearing methods such as sonication, streamlining the workflow and minimizing hands-on time.

The kit integrates DNA fragmentation and end-repair into a single-step reaction, significantly reducing overall library preparation time and cost. It is suitable for a broad range of sample types, including plant genomes, animal genomes, microbial genomes, and more, with a flexible DNA input range from 1 ng to 1 μg.

The optimized enzymatic fragmentation ensures consistent and uniform library fragment sizes across different species, minimizing variation and enhancing sequencing performance. The prepared libraries are fully compatible with Illumina™ or MGI™ adapters and primers, enabling seamless downstream sequencing on respective platforms.

Features

• Ultra-Simplified Pre-Mix

An all-in-one master mix streamlines library preparation with minimal pipetting and hands-on time.

• Broad Input Compatibility

Supports 1 ng–1 μg DNA inputs, including gDNA, full-length cDNA, and other DNA samples.

• Low-Bias Fragmentation

Delivers random DNA fragmentation with minimal sequence bias for accurate genome representation.

• Exceptional Library Performance

High-efficiency library conversion and high-fidelity amplification generate high-complexity libraries with robust yield for reliable downstream sequencing.

• Stable Pre-Mixed Formula 

Optimized pre-mixed formulation delivers outstanding stability, reproducible performance, and reduced hands-on variability.

Specifications

Components

Layout of the Pre-packaged, plate-based library prep kit format.

Storage

This product should be stored at -25~-15℃ for one year..

Application

DNA library Preparation; Whole genome sequencing(WGS); whole exome or other targeted capture sequencing; amplicon sequencing; immunoprecipitation sequencing; metagenomic sequencing;

Figures

1. Ultra-Simplified Pre-Mix 

 Figure 1. The ultra-simplified 3-tube pre-mix workflow of the EvoMax DNA Library Prep Kit.

2. Broad Species Compatibility

 Figure 2. Library uniformity testing across different species using 12972 under identical fragmentation conditions.

Different DNA samples were fragmented with 12972 under identical conditions. The libraries showed consistent sizes (~450 bp / ~320 bp) with good uniformity across species.

3. Strong Stability

 Figure 3. Stability assessment of the EvoMax DNA Library Prep Kit.

The library performance remained comparable to the control after 2 weeks at 4°C and 8 months of real-time stability at -20°C.

Documents:

Safety Data Sheet

12112_MSDS_HB260629

Manuals

12112_Manual_HB20260629