Product Alias

MHC, KRAS, K-Ras 2, KRAS2, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KRAS1, KRAS2, NS, NS3, RASK2, GTPase Kras, KI-RAS, RALD

Species

Human

Expression sequence

Gly25-Thr305 (HLA-A*11:01), Ile21-Met119 (B2M) and VVVGADGVGK peptide

Accession

AAV53343.1(HLA-A*11:01)&P61769(B2M)&VVVGADGVGK

Label

C-His-Avi

Expression system

E.coli

Molecular weight

The protein has a predicted MW of 35.36 kDa (HLA-A*11:01) and 11.9 kDa (B2M) same as SDS-PAGE result.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Endotoxin

< 1.0 EU per μg by the LAL method.

Preparation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Appearance

Lyophilized Powder

Biological Activity

【ELISA Data】 Immobilized HLA-A*11:01&B2M&KRAS G12D TCR at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag with the EC50 of 0.1μg/ml determined by ELISA.

Reconstitution method

Centrifuge before opening the lid. Reconstitute the lyophilized protein with distilled water to a concentration of more than 100 μg/mL. It is recommended to aliquot the protein into the amount required for each experiment when using it for the first time to avoid repeated freezing and thawing.