Beskrivning
Dendritic Cells (DCs) are the most effective antigen-presenting cells in the human body, serving as a bridge between innate immunity and adaptive immunity. However, their scarcity in vivo and the difficulty in direct acquisition greatly limit DC research and applications. Nevertheless, DCs can differentiate and be induced from DC precursor cells in various tissues, such as precursor cells in bone marrow fluid, and monocytes in peripheral blood and umbilical cord blood. For humans, since peripheral blood collection is the most convenient and yields the highest number of monocytes, the most common method is to induce DCs from human peripheral blood mononuclear cells (PBMCs). Currently, the most widely used method for generating human DCs is the induction of peripheral blood monocytes using a combination of GM-CSF and IL-4.
This cytokine set contains recombinant Human IL-4 and recombinant Human GM-CSF. These two cytokines are key components for culturing human dendritic cells. IL-4 can inhibit the differentiation of monocytes into CD14+ macrophages, thereby inducing their differentiation into immature DCs. Without IL-4 in the culture system, monocytes will differentiate into macrophages. GM-CSF can induce monocytes or DC precursor cells to differentiate into DCs and promote DC proliferation and survival. GM-CSF can also upregulate the expression of surface antigens on DCs, enhancing their antigen capture and processing capabilities. Human IL-4 acts synergistically with GM-CSF to jointly drive the differentiation process. Typically, in the presence of recombinant IL-4 and GM-CSF, monocytes can differentiate into immature dendritic cells, which can achieve full maturation upon further stimulation.
Product Information
|
Product Information. |
Catalog Number |
Size |
|
Human Dendritic Cell Differentiation Cytokine Set |
92644ES08 |
5 μg |
|
92644ES20 |
20 μg |
|
|
92644ES50 |
50 μg |
|
|
92644ES60 |
100 μg |
Components
|
Component Cat No. |
Component Name |
92644ES08 |
92644ES20 |
92644ES50 |
92644ES60 |
Quantity |
|
92644ES-A |
Recombinant Human GM-CSF Protein |
5 μg |
20 μg |
50 μg |
100 μg |
1 |
|
92644ES-B |
Recombinant Human IL-4 Protein |
5 μg |
20 μg |
50 μg |
100 μg |
1 |
Product Includes
|
Component Cat No. |
Product Name |
Source |
Appearance |
|
91102ES |
Recombinant Human GM-CSF Protein |
E.coli |
Lyophilized powder |
|
90105ES |
Recombinant Human IL-4 Protein |
E.coli |
Lyophilized powder |
Specifications
|
Parameter |
Specification |
|
Source |
Human |
|
Tag |
No Tag |
|
Expression System |
E. coli |
|
Purity |
> 98% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
< 1.0 EU/μg by the LAL method. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
|
Appearance |
Lyophilized Powder |
Reconstitution Method
|
Component Name |
Reconstitution Method |
|
Human GM-CSF Protein |
Before opening, centrifuge first to bring the contents to the bottom. Reconstitute with sterile water to a concentration of 0.1-1.0 mg/mL; further dilution can be performed according to subsequent experiments. For long-term storage, it is recommended to add carrier protein (0.1% BSA) to the dilution buffer; aliquot into single-use amounts, store frozen at -80°C, and avoid repeated freeze-thaw cycles. |
|
Human IL-4 Protein |
Storage
|
Component Name |
Storage Conditions |
|
Human GM-CSF Protein |
Store at -25 ~ -15°C, valid for 1 year from the date of receipt. After reconstitution, store at 2~8°C under sterile conditions, valid for 7 days. After reconstitution, store at -85 ~ -65°C under sterile conditions, valid for 3 months. |
|
Human IL-4 Protein |
Notes
1. For your safety and health, please wear a lab coat and disposable gloves during operation.
2. This product is for research use only.
Documents:
Manuals:
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