Beskrivning
Vascular Endothelial Growth Factor 165 (VEGF165) is a potent growth and angiogenic cytokine that stimulates endothelial cell proliferation and survival, promoting angiogenesis and vascular permeability. VEGF is expressed in vascularized tissues and plays an important role in both physiological and pathological angiogenesis. In stem cell differentiation, VEGF is a commonly supplemented growth factor used to direct the differentiation of pluripotent stem cells into hematopoietic progenitor cells. The VEGF family comprises multiple isoforms, among which VEGF165 is an isoform of VEGF-A and one of the most extensively studied.
Product Properties
|
Item |
Specification |
|
Synonyms |
VEGF-165, VEGF, VEGFA, MVCD1, VPF, RP1-261G23.1 |
|
Species |
Human |
|
Expressed Sequence |
Ala27-Arg191 |
|
Accession |
P15692-4 |
|
Tag |
No Tag |
|
Expression System |
CHO |
|
Molecular Weight |
The protein has a calculated MW of 19.2 kDa. The protein migrates as 24±3 kDa when calibrated against the Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
|
Purity |
> 95% as determined by SDS-PAGE. |
|
Endotoxin |
< 0.01 EU/μg protein as determined by LAL method. |
|
Formulation |
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with trehalose as protectant. |
|
Appearance |
Lyophilized Powder |
|
Biological Activity |
ED50 < 10 ng/mL, measured in a cell proliferation assay using HUVEC cells. |
|
Reconstitution Method |
It is recommended to reconstitute the protein in sterile PBS to a concentration of 0.1-1.0 mg/mL. Avoid repeated freeze-thaw cycles whenever possible. |
Features
- High purity: >95%, preventing immune reactions caused by residual host cell protein (HCP) or DNA.
- Ultra-low endotoxin: <10 EU/mg, minimizing the risk of fever or inflammation.
- Batch-to-batch consistency: ensuring stability and reproducibility in cell culture.
- Regulatory compliance: meeting regulatory requirements for cell therapy products and supporting IND submissions.
- Animal-origin-free: free of animal viruses, pathogenic agents, and adventitious agent contamination, ensuring high safety.
Regulatory Guideline Compliance
The manufacturing and quality control of GMP-grade proteins comply with the following systems and processes:
1. Complies with the General Principles of Volume III of the Pharmacopoeia of the People's Republic of China (2020 Edition): Quality Control of Raw Materials and Excipients for Biological Product Manufacturing.
2. Complies with USP Chapter <92> Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
3. Complies with Ph. Eur. General Chapter 5.2.12, Raw Materials of Biological Origin for the Production of Cell-based and Gene Therapy Medicinal Products.
Quality Management System
1. GMP proteins are manufactured, tested, and released under an ISO 13485:2016-certified quality management system, ensuring batch-to-batch consistency and material traceability.
2. Supplied with an approved Certificate of Analysis (COA).
3. Animal-origin-free materials and production environment.
4. Manufacturing site for pharmaceutical raw materials and excipients.
5. On-site and remote audits supported.
Product Safety Testing System
• Endotoxin: <10 EU/mg
• Residual host DNA: < 20 ng/mg
• Residual host cell protein: < 0.5 ng/μg
• Sterility test: Negative
• Mycoplasma test: Negative
• Adventitious virus test: Negative
Applications
SDS-PAGE
Figure 1. The purity is greater than 95% as determined by SDS-PAGE under reduced condition.
Bioactivity
Figure 2. The ED50 as determined by a cell proliferation assay using human HUVEC cells is less than 10 ng/mL.
Storage
Store at -25 to -15°C, valid for 3 years. After reconstitution, store at -85 to -65°C, valid for 3 months.
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