Beskrivning
This precast gel utilizes a Bis-Tris buffering system and features plastic cassettes, offering superior resolution with sharper, clearer bands. Compared to conventional Tris-Glycine systems, this precast gel provides stronger buffering capacity. It is used with a neutral running buffer, which enhances gel stability and prevents protein post-translational modifications during electrophoresis. A proprietary casting technology ensures excellent batch-to-batch consistency in both performance and band pattern reproducibility, delivering reliable electrophoresis results.
This series of precast gels includes both gradient and fixed-concentration formulations. The stacking gel height is 1.5 cm. Available gradient concentrations are 4–12% and 4–20%; fixed concentrations include 8%, 10%, and 12%. Each gel format offers either 10 or 15 wells: the maximum loading volume is 70 μL per well for 10-well gels and 40 μL per well for 15-well gels.
These gels are compatible with Yeasen’s PET 2 Mini Vertical Protein Electrophoresis System for two gels (Cat# 80210ES / 80212ES) and PET 4 Mini Vertical Protein Electrophoresis System for four gels (Cat# 80211ES / 80213ES) when used with the dedicated precast gel sealing strips. They are also compatible with most standard mini SDS-PAGE electrophoresis systems, including Bio-Rad Mini-PROTEAN (II/3/Tetra Systems), Tanon VE180 (note: install sealing strips in reverse orientation), and Life Technologies Novex Mini-Cell (used with custom spacers).
This precast gel is designed to be used with: Precast Running Buffer (Tris-MOPS), 2 L (Powder) (Cat# 36271ES).
Features
- User-Friendly: Ready-to-use—simply peel off the blue tape at the bottom of the gel cassette before use.
- High Gel Toughness: Cast under mildly acidic conditions, making the gel resistant to cracking during electrophoresis or staining.
- Sharp Bands: Plastic cassettes are specially treated to minimize protein adsorption during electrophoresis, ensuring clear, well-defined bands.
- Consistent Quality: Proprietary casting technology ensures excellent batch-to-batch reproducibility and uniform band patterns.
- Running buffer：50 mM Tris, 50 mM Mops, 0.1% SDS, 1 mM EDTA.
Components
|
Name |
36270ES10 |
|
Precast Protein Plus Gel, 4-20%, 15 wells, Bis-Tris |
10 gels / box |
Storage
This product should be stored at 2~8℃ for 1 year. Do not freeze below 0°C.
Figures
1. Migration Profile: Precast Protein Plus Gel (Bis-Tris)
2. Application Case-Sharp, Clear Bands
Figure 1. SDS-PAGE of E. coli lysates and protein markers on the Precast Protein Plus Gel (4-20%, 15-wells, Bis-Tris), showing sharp, clear bands and consistent separation.
Documents:
Safety Data Sheet
Manuals
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Produkten är endast avsedd för forskningsändamål och är inte avsedd för terapeutisk eller diagnostisk användning hos människor eller djur. Produkter och innehåll skyddas av patent, varumärken och upphovsrätt som ägs av Yeasen Biotechnology. Varumärkessymboler anger ursprungsland, inte nödvändigtvis registrering i alla regioner.
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