GoldBand Plus 3-color Regular Range Protein Marker (10-180 kDa) _ 26616ES

YeasenSku: 26616ES72

Size: 250 μL
Pris:
Försäljningspris$75.00
Stock:
I lager

GoldBand Plus 3-color Regular Range Protein Marker contains 10 highly purified and pre-stained recombinant proteins ranging from 10 kDa to 180 kDa (10, 15, 25, 35, 40, 55, 70, 100, 130, and 180 kDa), in which the 70 kDa band is orange-red, the 10 kDa band is green, and the remaining bands are blue. The indicated apparent molecular weights are calibrated against standard unstained protein molecular weight markers.

This product allows dynamic monitoring of protein electrophoresis status and transfer efficiency. After SDS-PAGE electrophoresis, clear colored bands are visible following transfer onto PVDF or NC membranes. Because pre-stained proteins are conjugated to dyes, the apparent molecular weights may vary in gels of different concentrations, as noted in the manual; this product is therefore for reference only when judging the molecular weight of target proteins. Yeasen provides a complete Western Blot solution; for related products, please refer to the WB product series selection guide.

Features

Seamless Compatibility: Band positions align perfectly with leading imported brands, allowing for a hassle-free, direct replacement in your existing workflows.

Vivid & Sharp Visualization: Three-color pre-staining delivers distinct, sharp bands with high brightness for easy interpretation.

Consistent Migration: The blue tracking dye remains highly stable across various running buffers, while the red and green dyes migrate predictably according to expected patterns.

High Sensitivity: Delivers clear, distinct results with a low loading volume of just 3–5 μL for standard mini-gels.

Superior Stability: Demonstrates excellent thermal stability with no degradation observed after 20 hours at 50°C.

Specifications

Cat.No.

Size

26616ES72

250 μL

26616ES76

2× 250 μL

26616ES90

10× 250 μL

Figures

Tris-Glycine running buffer

Figure 1. Protein marker migration profile under Tris-Glycine running buffer conditions

Figure 1. Protein marker migration profile under Tris-Glycine running buffer conditions

Performance Comparison

Figure 2. Performance comparison between 26616ES (Yeasen) and Supplier T in different buffer systems

Figure 2. Performance comparison between 26616ES (Yeasen) and Supplier T in different buffer systems

Apparent molecular weight of each band

Table 1. Apparent molecular weight of each band under different running buffer conditions

Table 1. Apparent molecular weight of each band under different running buffer conditions

Storage

Store at -15 to -25°C. Shelf life: 2 years. If used frequently, the product can be stored at 4°C for up to 3 months. Aliquotting is recommended to avoid repeated freeze-thaw cycles.

FAQ

Pre-stained protein markers from different manufacturers differ in the types of dyes used, labeling protocols, and buffer systems, which can lead to varying degrees of band migration shift (i.e., differences between apparent and actual molecular weights), and fully consistent results cannot be achieved. Please refer to the gel comparison images on our website. If precise determination of molecular weight is required, unstained protein markers can be used.
No. Because this product contains SDS, proteins are denatured, and their molecular weights are indicated under SDS-PAGE conditions: proteins bind to SDS and their intrinsic charges are masked, so migration depends only on molecular weight. In native PAGE, however, proteins retain their natural conformation and charge, and the migration rate is determined jointly by molecular size, shape, and charge; therefore, the migration position of proteins cannot be judged by molecular weight alone.
In theory, markers have no antibody binding sites and cannot be detected; however, if the target protein has binding sites similar to those of the marker, it may be recognized by the antibody and thereby visualized. Thermo Fisher has also provided relevant explanations regarding marker visualization. This possibility cannot be avoided.
This product allows dynamic observation of protein electrophoresis status and transfer efficiency. After SDS-PAGE electrophoresis and transfer onto PVDF or NC membranes, clear colored bands are visible.

Citations

Documents

Applications

Du kanske också gillar

Förfrågan

Vanliga frågor

Produkten är endast avsedd för forskningsändamål och är inte avsedd för terapeutisk eller diagnostisk användning hos människor eller djur. Produkter och innehåll skyddas av patent, varumärken och upphovsrätt som ägs av Yeasen Biotechnology. Varumärkessymboler anger ursprungsland, inte nödvändigtvis registrering i alla regioner.

Vissa applikationer kan kräva ytterligare immateriella rättigheter från tredje part.

Yeasen är dedikerad till etisk vetenskap, och anser att vår forskning bör behandla kritiska frågor samtidigt som den säkerställer säkerhet och etiska standarder.