Beskrivning
UltraNuclease, also known as non-specific nuclease or broad-spectrum nuclease, is a non-specific endonuclease derived from Serratia Marcescen. It can cleave at any nucleotide within the chain, completely digesting nucleic acids into 5'-monophosphate oligonucleotides of 2-5 bases in length. UltraNuclease is capable of degrading various forms (double-stranded, single-stranded, linear, circular, native, or denatured) of DNA and RNA under a wide range of conditions (6 M Urea, 0.1 M Guanidine HCl, 0.4% Triton X-100, 0.1% SDS, 1 mM EDTA, 1 mM PMSF). It is widely used for removing nucleic acids from biological products.
This product is genetically engineered and expressed in Escherichia coli (E. coli), with a purity ≥99%. It is used to reduce the viscosity of cell supernatants and cell lysates, improve protein purification efficiency and functionality studies, and effectively prevent the clumping of human peripheral blood mononuclear cells (PBMCs) in cell therapy and vaccine research.
It is provided as a sterile liquid enzyme stored in buffer (20 mM Tris-HCl pH 8.0, 2 mM MgCl2, 20 mM NaCl, 50% Glycerol), appearing as a clear, colorless liquid.
Features
Wide range of applications: degrade all forms of DNA and RNA
High purity and activity: purity ≥ 99%; specific activity ≥ 1.5×106 U/mg
Specification
|
Source |
E. coli |
|
Molecular Weight |
26.5 kDa |
|
Isoelectric Point |
6.85 |
|
Purity |
≥ 99%（HPLC） |
|
Enzyme Activity |
250-300 U/μL |
|
Specific Activity |
≥1.5×106 U/mg protein |
|
Optimum pH |
8.0 (pH range 6-10) |
|
Optimum Temperature |
37°C (temperature range 0-42°C) |
|
Cofactor |
1-10 mM Mg2+ |
|
Storage Buffer |
20 mM Tris-HCl pH8.0，2 mM MgCl2，20 mM NaCl，50% Glycerol |
|
Unit Definition |
One unit (U) of enzyme activity is defined as the amount of enzyme required to cause an increase in absorbance (ΔA260) of 1.0 in 30 minutes under reaction conditions of 37°C, pH 8.0 in a total reaction volume of 2.625 mL, equivalent to the complete digestion of 37 μg of salmon sperm DNA into oligonucleotides. |
Components
|
Name |
20156ES25 |
20156ES50 |
20156ES60 |
|
UCF.METM UltraNuclease |
25 KU |
50 KU |
100 KU |
Storage
Store Part I components at -25°C ~ -15°C; store Part II components at 2~8°C;
store Part III components at room temperature. The product is valid for 1 year.
Application
- Purification of viral vaccines, viral vectors for the vaccine, and oncolytic viruses;
- Protein purification-Efficient removal of contaminating DNA and RNA from proteins and other biologics;
- Reduction of viscosity caused by nucleic acids;
- Sample preparation in electrophoresis and chromatography;
- Prevention of cell clumping.
Figures
1. High Purity
Figure 1. Purity analysis of the enzyme. (A) SDSPAGE showing ≥ 99% purity; (B) HPLC profile showing ≥ 99% purity.
2. Enzymatic activity testing under different conditions
Figure 2. Single‑factor comparison of Universal Nuclease activity.
No significant difference was observed between this product and Supplier M under identical test conditions.
3. Application Examples
Documents:
Safety Data Sheet
Manuals
20156_Manual_Ver.EN20250806.pdf
Citations & References:
[1] Lin J, Chen Z, Yang L, et al. Cas9/AAV9-Mediated Somatic Mutagenesis Uncovered the Cell-Autonomous Role of Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2 in Murine Cardiomyocyte Maturation. Front Cell Dev Biol. 2022;10:864516. Published 2022 Apr 1. doi:10.3389/fcell.2022.864516(IF:6.684)
[2] Kang D, Liu Y, Song Y, Fang B, Zhang Q, Hu L. Triptolide Shows High Sensitivity and Low Toxicity Against Acute Myeloid Leukemia Cell Lines Through Inhibiting WSTF-RNAPII Complex. Front Oncol. 2022;12:811850. Published 2022 Feb 16. doi:10.3389/fonc.2022.811850(IF:6.244)
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