Beskrivning
The Hieff™ Magnetic Circulating Cell-Free DNA Kit is suitable for extracting circulating DNA from 0.3-4 ml human plasma and serum. This product adopts unique magnetic beads and carefully optimized buffer system, which can maximize the purification and recovery of circulating DNA (typically 1-100 ng/ml circulating DNA in human plasma). The extracted nucleic acid is suitable for various downstream applications, such as NGS, qPCR, etc.
Feature
High Yield: Delivers high nucleic acid concentration with excellent recovery of short fragments.
Superior Quality: Produces high-quality libraries for downstream sequencing.
Broad Compatibility: Works with 0.3–4 mL plasma samples from both cfDNA-specific and standard EDTA tubes.
Components
|
Components No. |
Contents |
18386ES12 |
18386ES48 |
|
|
12 T |
48 T |
|||
|
Part I |
18386-A |
Proteinase K (20 mg/mL) |
1.25 mL×2 |
10 mL |
|
18386-B |
Magnetic Bead Suspension |
750 μL ×2 |
6 mL |
|
|
Part II |
18386-C |
Lysis Buffer |
2.5 mL |
10 mL |
|
18386-D |
Binding Buffer |
65 mL |
125 mL ×2 |
|
|
18386-E |
Wash Buffer A |
80 mL |
320 mL |
|
|
18386-F |
Wash Buffer B* |
22 mL |
88 mL |
|
|
18386-G |
Elution Buffer |
5 mL |
15 mL |
|
Storage
Both components can be transported at room temperature. For storage, Component I should be kept at 2–8°C, while Component II can be stored at room temperature. Both have a shelf life of 18 months.
Figure
Case Study 1: Superior cfDNA Recovery from Healthy Donor Plasma
Using a 50 µL elution volume, the 18382 kit demonstrated outstanding recovery efficiency from 2 mL of healthy donor plasma collected in cfDNA preservation tubes. Quantitative analysis confirmed that the kit delivers high nucleic acid yields, ensuring sufficient template material for sensitive downstream molecular applications.
|
Sample |
Competitor N (Conc. ng/µL) |
Supplier N (Total ng) |
18382 (Conc. ng/µL) |
18382 (Total ng) |
|
2 mL Healthy Plasma-1 |
0.296 |
14.80 |
0.437 |
21.85 |
|
2 mL Healthy Plasma-2 |
0.262 |
13.10 |
0.308 |
15.40 |
|
2 mL Healthy Plasma-3 |
0.378 |
18.90 |
0.410 |
20.50 |
|
2 mL Healthy Plasma-4 |
0.525 |
21.00 |
0.600 |
24.00 |
|
Average |
0.365 |
16.95 |
0.439 |
20.44 |
Case Study 2: High-Integrity cfDNA Extraction from EDTA Plasma
Using a 20 µL elution volume, the 18382 kit achieved excellent recovery efficiency from 2 mL of healthy donor EDTA plasma. Fragment analysis revealed distinct characteristic peaks at 170 bp and 340 bp, confirming the high integrity of the extracted cfDNA. Notably, no high-molecular-weight DNA interference was observed, ensuring high-purity nucleic acids ready for downstream sequencing or PCR assays.
Figure 1. cfDNA yield and fragment analysis from 2 mL EDTA plasma using the 18382 kit.
Documents:
Safety Data Sheet
Manuals
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Förfrågan
Vanliga frågor
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