SP6 Bacteriophage RNA Polymerase is a DNA-dependent RNA polymerase that exhibits high specificity for the SP6 bacteriophage promoter. It catalyzes the incorporation of NTPs downstream of the SP6 promoter on single- or double-stranded DNA templates, synthesizing RNA complementary to the DNA template downstream of the SP6 promoter. This enzyme not only performs conventional RNA synthesis but also recognizes modified NTPs, such as biotin-, digoxigenin-, or fluorescein-labeled NTPs, making it suitable for a wide range of research applications and biotechnological uses.

Features

High Purity: Free from exonuclease, endonuclease, non-specific nuclease, and RNase contamination, ensuring clean and reliable transcription.

High Transcription Efficiency: Delivers robust in vitro RNA synthesis performance comparable to leading international brands.

Recombinant Source: Produced in recombinant E. coli expressing the Salmonella typhimurium SP6 RNA polymerase gene.

Defined Promoter Recognition: Recognizes the SP6 promoter sequence (5′-ATTTAGGTGACACTATAGAAGNG-3′) for precise and efficient transcription initiation.

Components

Application

Preparation of radiolabeled RNA probes

Non-isotopic RNA labeling

RNA vaccine preparation

Guide RNA for target genes

mRNA for in vitro translation and microinjection

RNA for structural, processing, and catalytic studies

RNA amplification

Synthesis of RNA antisense strands for gene expression regulation

Figures

1. High Transcription Efficiency

Figure 1. In vitro transcription efficiency of Yeasen SP6 RNA Polymerase compared with a leading brand.

In vitro transcription was performed using a linearized plasmid DNA template containing the SP6 promoter. Reactions included varying amounts of Yeasen SP6 RNA Polymerase or a leading brand's enzyme. After incubation at 37°C for 2 h, reactions were treated with DNase I to remove template DNA. The resulting 248 nt RNA transcripts were analyzed by 1% agarose gel electrophoresis in 1× TAE buffer and visualized by staining.

2. High Purity

Figure 2. Purity assessment of Yeasen SP6 RNA Polymerase for exonuclease, nickase, nonspecific nuclease, and RNase contamination.

SP6 RNA Polymerase was incubated with nucleic acid substrates and analyzed by agarose gel electrophoresis to observe any band changes. The results demonstrate that all three batches of Yeasen SP6 RNA Polymerase tested negative for exonuclease (100 U), nickase (100 U), nonspecific nuclease (100 U), and RNase (20 U) contaminants. This ensures the accuracy and reliability of your experimental outcomes.