Hieff Unicon™ Hotstart J-Taq DNA Polymerase, Glycerol-free (40 U/μL) is a hot-start DNA polymerase. This product utilizes antibody-mediated inhibition, providing high amplification sensitivity and specificity. The inhibitory antibody is completely inactivated and the DNA polymerase activity is fully released after a 30-second heat activation step at the initial denaturation temperature. Using this hot-start Taq enzyme effectively suppresses non-specific amplification caused by primer mis-annealing. This glycerol-free formulation is specifically designed for lyophilized reagent preparation.

Features

Rapid Activation: Taq polymerase achieves full activity after a quick 30 s incubation at 95 °C, ensuring immediate and efficient amplification.

High Sensitivity & Specificity: Provides strong amplification performance even with low template input, minimizing non-specific products.

Broad Instrument Compatibility: Works seamlessly with a wide range of standard PCR and real-time PCR platforms.

Lyophilization-Ready: Formulation supports freeze-drying, enabling long-term storage, reduced shipping costs, and simplified reagent handling.

Components

Application

Gene Expression Analysis; Low-Copy Target Amplification; Lyophilized PCR/qPCR.

Figures

1. Consistent Performance & Strong Stability

Figure 1. Stability of Yeasen glycerol-free enzyme (14326ES).

The glycerol-free enzyme showed the same amplification performance as the glycerol-containing enzyme across COVID-19 pseudovirus templates (105–103 copies/mL). After 54 days at 37 °C, its performance remained unchanged, confirming excellent thermal stability.