Описание
|
Cat# |
Size |
|
|
Recombinant Human IGF-1 Protein
|
92211ES10 |
10 μg |
|
92211ES60 |
100 μg |
|
|
92211ES76 |
500 μg |
|
|
92211ES80 |
1 mg |
IGFs are mitogenic peptide growth factors that stimulate the proliferation and survival of a variety of cell types in vitro, including muscle, bone, and cartilage tissues.IGFs are produced primarily by the liver, although a variety of tissues produce IGFs at different times.IGFs belong to the insulin gene family, which also contains insulin and relaxin.IGFs are structurally and functionally similar to insulin but have a much much higher growth-promoting activity.The expression of IGF-II is influenced by placental prolactin, whereas the expression of IGF-I is regulated by growth hormone.Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but IGF-II can also signal through the IGF-II/mannose-6-phosphate receptor. Mature IGFs are produced by proteolytic processing of inactive precursor proteins that contain N-terminal and C-terminal prepeptide regions.
This product, Recombinant Human IGF-1, is supplied as a lyophilized powder with high activity, high purity and low endotoxin.
Specification
|
Synonyms |
Somatomedin C, IGF-I, IGF-IA, Mechano growth factor, MGF |
|
Source |
E.coli-derived human IL-IGF-1 protein, Gly49-Ala118 without tag. |
|
Accession |
P05019 |
|
Molecular Weight |
Approximately 7.6 kDa. |
|
AA Sequence |
GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA |
|
Endotoxin |
< 0.01 EU/μg by the LAL method. |
|
Purity |
> 97%, SDS-PAGE and HPLC detection |
|
Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg. |
|
Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.0. |
|
Physical Appearance |
White lyophilized powder |
Storage
-25 ~ -15 ° C storage, valid for 1 year after receipt.
After re-solution, store at 2 ~8 °C, 30 days expiration date. After re-dissolving, store at -85~-65°C, 3 months expiration date.
Compounding Methods
Centrifuge before uncapping to bring contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL, which can be diluted even further according to subsequent experiments; for long-term storage, it is recommended that carrier proteins (0.1% BSA) be added to the dilution buffer; dispense for a single experimental dose and freeze at -80°C to avoid repeated freezing and thawing.
Cautions
1.For your safety and health, please wear lab coat and disposable gloves.
2.This product is for scientific research purposes only.
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Расследование
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