Описание
|
Product Name |
Cat# |
Size |
|
Recombinant Human IL-21 Protein |
90215ES05 |
2 μg |
|
90215ES10 |
10 μg |
|
|
90215ES50 |
50 μg |
|
|
90215ES60 |
100 μg |
|
|
90215ES80 |
1 mg |
IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation, and its effects typically enhance the antigen-specific response of immune cells.The biological effects of IL-21 include: inducing T cell-stimulated B cells to differentiate into plasma cells and memory B cells; combining with IL-4 to stimulate IgG production; and, in the absence of T cell signaling, inducing apoptotic effects in naïve B cells and stimulated B cells to induce apoptotic effects. In addition, IL-21 promotes the antitumor activity of CD8+ T cells and NK cells.IL-21 exerts its effects by binding to a specific type I cytokine receptor, IL-21R, which also contains the gamma chain (γc) found in other cytokine receptors, including IL-2, IL-4, IL-7, IL-9, and IL-15.IL-21/IL -21R interaction triggers a cascade of events, including activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3.
This product, Recombinant Human IL-21, is supplied as a lyophilized powder with high activity, high purity and low endotoxin.
Specification
|
Synonyms |
Interleukin-21; IL21; Za11 |
|
Source |
E.coli-derived human IL-21 protein, Gln30-Ser162 without tag. |
|
Accession |
|
|
Molecular Weight |
Approximately 15.4 kDa. |
|
AA Sequence |
QGQDRHMIRM RQLIDIVDQL KNYVNDLVPE FLPAPEDVET NCEWSAFSCF QKAQLKSANT GNNERIINVS IKKLKRKPPS TNAGRRQKHR LTCPSCDSYE KKPPKEFLER FKSLLQKMIH QHLSSRTHGS EDS |
|
Endotoxin |
< 1.0 EU/μg by the LAL method. |
|
Purity |
> 97% by SDS-PAGE and HPLC. |
|
Biological Activity |
Fully biologically active when compared to standard. Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is ≤ 2 ng/mL. The specific activity of recombinant human IL-21 > 5.0 × 105 U/mg. |
|
Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
|
Physical Appearance |
White lyophilized powder |
Storage
-25 ~ -15 ° C storage, valid for 1 year after receipt.
After re-solution, store at 2 ~8 °C, 30 days expiration date. After re-dissolving, store at -85~-65°C, 3 months expiration date.
Compounding Methods
Centrifuge before uncapping to bring contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL, which can be diluted even further according to subsequent experiments; for long-term storage, it is recommended that carrier proteins (0.1% BSA) be added to the dilution buffer; dispense for a single experimental dose and freeze at -80°C to avoid repeated freezing and thawing.
Cautions
1. Please operate with lab coats and disposable gloves,for your safety.
2. This product is for research use only.
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Расследование
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