Recombinant Alkalitolerant Protein A (Freeze-Dried) _ 20543ES
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Recombinant Alkalitolerant Protein A (Freeze-Dried) _ 20543ES
Recombinant Alkalitolerant Protein A (Freeze-Dried) _ 20543ES
Protein A is a cell wall component produced by several strains of Staphylococcus aureus, consisting of a polypeptide chain and containing almost no carbohydrates. This product is produced in Escherichia coli and functions similarly to natural protein A. It consists of five IgG binding domains E-D-A-B-C arranged in series, with strong specific affinity for the Fc fragment of IgG. Protein A, as an affinity ligand, is coupled to the matrix and can specifically bind to antibody molecules in the sample, allowing other impurities to flow through and exhibiting high selectivity. Commonly used for purification of...

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Recombinant Alkalitolerant Protein A (Freeze-Dried) _ 20543ES

YeasenSku: 20543ES10
Size: 10 mg
Цена:
$90.00
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В наличии
Protein A is a cell wall component produced by several strains of Staphylococcus aureus, consisting of a polypeptide chain and containing almost no carbohydrates. This product is produced in Escherichia coli and functions similarly to natural protein A. It consists of five IgG binding domains E-D-A-B-C arranged in series, with strong specific affinity for the Fc fragment of IgG. Protein A, as an affinity ligand, is coupled to the matrix and can specifically bind to antibody molecules in the sample, allowing other impurities to flow through and exhibiting high selectivity. Commonly used for purification of monoclonal antibodies or polyclonal antibodies. Protein A binds to human, mouse, and rat IgG subclasses (e.g. human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2c), as
well as total IgG from rabbits, guinea pigs, pigs, dogs, and cats.

Components

Components No.

Name

20543ES10

20543ES60

20543ES74

20543ES80

20543ES92

20543

Recombinant Alkalitolerant Protein A

10 mg

100 mg

400 mg

1 g

10 g

Product Properties

Synonyms

Recombinant protein A alkali-tolerant

Source

E.coli

Molecular Weight

Approximately 26.7 kDa, a single non-glycosylated polypeptide chain containing 237amino acids

Amino Acid Sequence

AQGTVDAKFDKEQQNAFYEILHLPNLTEEQRNAFIQSLKDDPSQSANLLAEAKKLND

AQAPKVDAKFDKEQQNAFYEILHLPNLTEEQRNAFIQSLKDDPSQSANLLAEAKKLN DAQAPKVDAKFDKEQQNAFYEILHLPNLTEEQRNAFIQSLKDDPSQSANLLAEAKKL NDAQAPKVDAKFDKEQQNAFYEILHLPNLTEEQRNAFIQSLKDDPSQSANLLAEAKK LNDAQAPKC

Concentration for 1 unity OD at 280 nm

4.4 mg/mL

Physical Appearance

> 97 % by SDS-PAGE and HPLC analyses.

Purity

> 97% by SDS-PAGE and HPLC analyses.

Endotoxin Level

<0.1 EU /1 μg of the protein by the LAL method.

Formulation

A 0.2 µm filtered solution in 20 mM PB,400 mM NaCl, pH7.4 or lyophilized from a 0.2 µm filtered solution in 20 mM PB,400 mM NaCl, pH7.4

Reconstitution

Dissolve in distilled water or saline.

Notes

1. Please operate with lab coats and disposable gloves ，for your safety.

2. This product is for research use only.

Appendix

Table 1. Summary of Binding Abilities of Proteins A, G, and L to Ig from Different Species

Table 1. Summary of&nbsp;Binding Abilities of&nbsp;Proteins&nbsp;A,&nbsp;G,&nbsp;and&nbsp;L&nbsp;to&nbsp;Ig&nbsp;from&nbsp;Different&nbsp;Species

Storage

This product should be stored at -25~ -15℃, valid for 12 months. Avoid repeated freeze-thaw cycles.

FAQ

Citations

Documents

Manual
20543-Manual-Ver.EN20250701.pdf
MSDS
20543-MSDS-HB250701.pdf

Applications

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