Hieff™ Bacterial RNA Kit _ 19301ES
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Hieff™ Bacterial RNA Kit _ 19301ES
Hieff™ Bacterial RNA Kit _ 19301ES
This Kit is designed for rapid and efficient RNA extraction from Gram-positive and Gram-negative bacteria.Utilizing a proprietary silica- membrane column technology combined with optimized buffers, this kit effectively removes contaminants such as proteins, metabolites, and genomic DNA. The purified RNA is suitable for downstream applications including RT-PCR, RT-qPCR, in vitro transcription, and molecular cloning. Specifications Cat.No. 19301ES50 Size 50 T Components Category Components No. Name 19301ES50 Part I 19301-A Lysozyme 20 mg Part II 19301-B DNA-Removing Column B4 50 units 19301-C Hieff™ RNA Column B4 50 units 19301-D 2 mL Collection Tube B4 100 units 19301-E Lysis Buffer(LB Buffer B4) 25 mL 19301-F...

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Hieff™ Bacterial RNA Kit _ 19301ES

YeasenSku: 19301ES50
Size: 50 T
Цена:
$210.00
Запас:
В наличии

This Kit is designed for rapid and efficient RNA extraction from Gram-positive and Gram-negative bacteria.Utilizing a proprietary silica- membrane column technology combined with optimized buffers, this kit effectively removes contaminants such as proteins, metabolites, and genomic DNA. The purified RNA is suitable for downstream applications including RT-PCR, RT-qPCR, in vitro transcription, and molecular cloning.

Specifications

Cat.No.

19301ES50

Size

50 T

Components

Category

Components No.

Name

19301ES50

Part I

19301-A

Lysozyme

20 mg

Part II

19301-B

DNA-Removing Column B4

50 units

19301-C

Hieff™ RNA Column B4

50 units

19301-D

2 mL Collection Tube B4

100 units

19301-E

Lysis Buffer(LB Buffer B4)

25 mL

19301-F

PL Buffer B4

40 mL

19301-G

Wash Buffer B4*

13 mL(add 52 mL ethanol before use)

19301-H

TE Buffer pH 8.0

6 mL

19301-I

RNase-free H2O

5 mL

Features

Wide applicability: can be used for bacterial cultures such as Escherichia coli, Pseudomonas aeruginosa, and Gram-positive bacteria.

Quick and easy: the operation can be completed within 40 minutes.

High RNA purity: DNase I on-column digestion effectively removes gDNA.

Safe and environmentally friendly: no need to use organic matter such as phenol and chloroform for extraction.

Applications

Bacterial RNA extraction；RNA extraction from Gram-positive bacteria；Gram-negative bacteria, etc.

Figures

Suppliers

Concentration

OD260/OD280

OD260/OD230

Yeasen-19031ES

159.40/193.18

1.895/1.925

2.177/2.101

Supplier T*

182.01/179.31

1.855/1.922

0.728/1.206

Table 1. E. coli extract product concentration

Figure 1. Electrophoresis of E. coli RNA extraction products

Figure 1. Electrophoresis of E. coli RNA extraction products

Take 4 portions of 2 mL of E. coli suspension, use 19301 and similar products from supplier T brand to extract total RNA, and elute with 50 ul RNase-free H2O. Then take 1 ul of the eluate to determine the concentration. Take 5 ul of the eluate to identify the RNA integrity and band brightness. The results show that the 19301 extraction product has a high yield and good RNA integrity.

Storage

Part I components should be stored at 4~8℃ for 2 years.

Part II components should be stored at room temperature for 2 years.

FAQ

Citations

Documents

Manual
19301ES-Manual-_Ver.EN20250825.pdf
MSDS
19301ES--MSDS-HB250516_5edc09ca-df23-4c37-b3ed-57f98d582d91.pdf

Applications

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