Описание
The RT-LAMP Fluorescent Assay Kit (Bst Plus) is a kit capable of isothermal amplification using DNA and RNA as templates. This kit employs the next-generation aptamer-encapsulated Bst Plus DNA Polymerase and heat-resistant HifairTM III Reverse Transcriptase, along with an MRI, combined with a finely optimized buffer system, to achieve highly efficient, rapid, and highly specific detection reactions, and supports room-temperature reaction system preparation.
Features
- Broad Compatibility: Compatible with over 20 animal and human pathogen targets, with GC content ranging from 30% to 65%. Delivers comparable amplification times to leading products.
- High Specificity: Validated across diverse DNA and RNA targets. Demonstrates superior specificity with no false positives within 60 minutes and delayed amplification in NTCs compared to competitors.
- Excellent Sensitivity: Capable of detecting African swine fever virus at 15 copies/reaction and Influenza B virus at 10 copies/reaction.
- Robust Stability: Maintains performance after 20 freeze-thaw cycles and 7 days at 37°C. Supports convenient room temperature reaction setup.
Components
|
Components No. |
Name |
16729ES60 |
16729ES80 |
|
16729-A |
4×LAMP Buffer |
600 µL |
6 mL |
|
16729-B |
Bst Plus DNA Polymerase |
100 µL |
1 mL |
|
16729-C |
RT Enzyme mix |
50 µL |
500 µL |
|
16729-D |
25×Fluorescent Dye |
100 µL |
1 mL |
Shipping and Storage
This product should be stored at -25~-15℃ for 1 year.Component D must be stored away from light.
Application
Isothermal Amplification;Rapid detection of pathogenic bacteria.
Figures
1. Workflow
Figure 1. RT-LAMP Workflow.
2. Broad Compatibility: DNA Pathogen Detection&RNA Pathogen Detection
Figure 2. Broad Pathogen Detection Compatibility.
(A) DNA pathogen detection: Reliable amplification observed across multiple DNA virus targets.
(B) RNA pathogen detection: Effective detection of RNA viruses with high sensitivity and specificity.
3. High Specificity
Figure 3. High Specificity of RT-LAMP Assay (Cat#16729) Compared to Supplier A*
Specificity was evaluated using both DNA and RNA templates, including positive targets and non-target (negative) controls. The RT-LAMP assay (Cat#16729) showed no false positives and clear separation between positive and negative samples, outperforming Supplier A* by avoiding non-specific amplification.
4. Strong stability
Figure 4. Stability Evaluation of the RT-LAMP System.
(A) Freeze-thaw stability: Performance remains consistent after 20 freeze-thaw cycles.
(B) Thermal accelerated stability: Maintains amplification efficiency after 7 days of storage at 37°C.
Documents:
Safety Data Sheet
Manuals
16729_Manual_Ver.EN20250708.pdf
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Расследование
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