Description
IL-1 Receptor Accessory Protein (IL-1 RAcP), also known as IL-1 R3, is a ubiquitously expressed 70-90 kDa member of the Interleukin-1 receptor family of proteins . It serves as a non-ligand-binding component of the receptors for IL-1 alpha, IL-1 beta, IL-33, and IL-36.
Specifications
|
Cat.No. |
96267ES25 / 96267ES60 / 96267ES76 |
|
Size |
25 μg / 100 μg / 500 μg |
Components
|
Name |
96267ES25 |
96267ES60 |
96267ES76 |
|
Recombinant Cynomolgus IL-1R3/IL-1 RAcP Protein,His Tag,Ultra Low Endotoxin |
25 μg |
100 μg |
500 μg |
Performance parameters
|
Synonyms |
IL1RAP;IL-1RAP;C3orf13;IL-1 R3;IL-1 RAcP |
|
Species |
Cynomolgus |
|
Expression Sequence |
Ser21-Cys362 |
|
Accession |
G7NYP7 |
|
Tag |
C-His |
|
Expression System |
HEK293 |
|
Molecular Weight |
The protein has a predicted MW of 40.49 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and SEC-HPLC |
|
Endotoxin |
Less than 0.01 EU per μg by the LAL method. |
|
Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Biological activity |
ELISA Data 1:Immobilized Cynomolgus IL-1R3, His Tag at 0.5 μg/mL (100 μL/well) on the plate. Dose response curve for Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 2.6 ng/ml determined by ELISA . |
|
Reconstitution Method |
Dissolve the lyophilized protein in distilled water. For vials containing 25 μg or 100 μg, we recommend reconstituting to a concentration of 0.5 mg/mL. For larger quantities (500 μg or 1 mg), a concentration of 1 mg/mL is suggested. During reconstitution, avoid vigorous vortexing or shaking. Aliquot the reconstituted solution as needed and store at -80°C. Avoid repeated freeze-thaw cycles. |
Storage
Store at -25 to -15°C for up to 1 year from the date of receipt; once reconstituted, store at -85 to -65°C where it remains stable for 3 months, avoiding repeated freeze-thaw cycles.
Notes
1. This product is for research use only.
2. Please operate with lab coats and disposable gloves,for your safety.
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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