Description
This kit contains Recombinant Human IL-2, Human IL-12, and Human IL-15. It is designed for the isolation, activation, and expansion of human Natural Killer (NK) cells from healthy human peripheral blood mononuclear cells (PBMCs).
IL-2: A T-cell growth factor that also effectively promotes NK cell proliferation and activation. It enhances NK cell cytotoxicity (perforin and granzyme expression); however, prolonged exposure to high concentrations of IL-2 may lead to activation-induced cell death and the expansion of Treg cells.
IL-15: The natural survival and homeostatic factor for NK cells. In in vitro culture, it plays a key role in maintaining basal NK cell survival, preventing apoptosis, and sustaining proliferation.
IL-12: A potent pro-inflammatory cytokine. It strongly induces IFN-γ production in NK cells and promotes the formation of "memory-like" or "adaptive" NK cells.
Components
|
Components No. |
Name |
92652ES10 |
92652ES50 |
92652ES60 |
|
92652ES-A |
Human IL-2 Protein |
10 μg |
50 μg |
100 μg |
|
92652ES-B |
Human IL-15 Protein |
10 μg |
50 μg |
100 μg |
|
92652ES-C |
Human IL-12 Protein |
10 μg |
50 μg |
100 μg |
Storage
Storage: Store at -25°C to -15°C. Valid for 1 year from the date of receipt.
After Reconstitution: Store at -85°C to -65°C under sterile conditions. Valid for 3 months.
Product Information
|
Original Cat. No. |
Product Name |
Source |
Appearance |
|
90213ES |
Human IL-2 Protein |
E.coli |
Lyophilized Powder |
|
90113ES |
Human IL-15 Protein |
E.coli |
Lyophilized Powder |
|
90262ES |
Human IL-12 Protein |
CHO Cells |
Lyophilized Powder |
Notes
1. This product is for research use only.
2. Please operate with lab coats and disposable gloves，for your safety.
Instructions
|
Component Name |
Reconstitution Method |
|
Human IL-2 Protein |
Centrifuge the vial prior to opening to collect contents at the bottom. Reconstitute with sterile deionized water to a concentration of 0.1-1.0 mg/mL. For subsequent experiments, further dilutions can be made. For long-term storage, it is recommended to add a carrier protein (0.1% BSA) to the dilution buffer. Aliquot into single-use volumes, store at -80°C, and avoid repeated freeze-thaw cycles. |
|
Human IL-15 Protein |
|
|
Human IL-12 Protein |
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.