Description
Leptin is a protein product of the mouse obese gene . It modulates multiple functions including energy homeostasis, thermoregulation, bone metabolism, endocrine, and pro-inflammatory immune responses. As a signal molecule with growth factor characteristics, leptin can stimulate osteoblastic cells and inhibit osteoclast formation and activity, thus promoting osteogenesis.
Product Properties
|
Synonyms |
Obese Protein, Obesity Factor |
|
Accession |
P41159 |
|
GeneID |
3952 |
|
Source |
E.coli-derived Human Leptin, Val22-Cys167. |
|
Molecular Weight |
Approximately 16.0 kDa. |
|
Tag |
None |
|
Purity |
> 95% by SDS-PAGE. |
|
Biological Activity |
Immobilized Human Leptin Antibody at 3 μg/mL (100 μL/well) can bind Human Leptin , the EC50 of Human Leptin is 174-246 pg/mL. |
|
Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4, 1%BSA. |
|
Reconstitution |
1.We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS, pH 7.4, with 0.1% BSA to a concentration of 0.1-1.0 mg/mL. 2.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. 3.Further dilutions should be made in PBS, which must contain carrier proteins, such as 0.1% BSA, 10% FBS, 5% HSA, 5% trehalose, one of four options. 5% trehalose is recommended for experimental culture without serum. |
Shipping and Storage
The products are shipped with dry ice and can be stored at -25~-15℃ for 1 year.
1 month, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ °C under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1.For your safety and health, please wear lab coats and disposable gloves for operation.
2.For research use only!
Assay Data
HB23020