Recombinant Mouse RSPO1 Protein, His Tag _ 92277ES

SKU: 92277ES60

Size: 100 μg
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Description

R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3. Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids .

 

Product Properties

 

Synonyms

R-Spondin 1;Roof Plate-specific Spondin 1Cristin3; R-spondin-1

Source

Expressed in E. coli.

Molecular Weight

Approximately 20.9kDa, a single non-glycosylated polypeptide chain containing 189 amino acid

Accession

Q9Z132Ser21-Gly209

Biological Activity

Fully biologically active when compared to standard. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 50-200 ng/mL in the presence of 5ng/mL recombinant mouse Wnt-3a.

Purity

> 96% by SDS-PAGE.

Endotoxin

0.01EU/μg protein as determined by LAL method.

Formulation

Lyophilized from a 0.2mm filtered solution in 20mM PB, 150mM NaCl, pH 7.4.

Reconstitution

Before use this product, please read the direction below carefully.

1.This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.

2.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.

Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

 

The product should be stored at -25~-15℃ for 1 year from date of receipt.

1 month, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~-15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

 

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

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