Description
All three isoforms of GRO are CXC chemokines that can signal through the CXCR1 or CXCR2 receptors. The GRO proteins chemoattract and activate neutrophils and basophils. Recombinant Human GRO-β is a 7.9 kDa protein consisting of 73 amino acids, including the 'ELR' motif common to the CXC chemokine family that binds to CXCR1 or CXCR2.
Product Properties
Synonyms |
MIP2-alpha |
Accession |
|
GeneID |
2920 |
Source |
E.coli-derived Human GRO-β Ala35-Asn107. |
Molecular Weight |
Approximately 7.9 kDa |
AA Sequence |
APLATELRCQ CLQTLQGIHL KNIQSVKVKS PGPHCAQTEV IATLKNGQKA CLNPASPMVK KIIEKMLKNG KSN |
Tag |
None |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity |
> 97% by SDS-PAGE and HPLC analyses. |
Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is in a concentration range of 10-100 ng/mL. |
Endotoxin |
< 1 EU per 1μg of the protein by the LAL method. |
Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1.Avoid repeated freeze-thaw cycle.
2.For your safety and health, please wear lab coats and disposable gloves for operation.
3.For research use only.