Description
IL-27 (Interleukin 27) is a cytokine belonging to the IL-12 family, composed of an EBI3 and a p28 subunit forming a heterodimeric cytokine. IL-27 is produced by activated dendritic cells and macrophages in response to Toll-like receptor ligands and pro-inflammatory cytokines. IL-27 promotes the differentiation of CD4+ T cells into the Th1 lineage by inducing the expression of the transcription factor T-bet and upregulating IL-12Rβ2, which in turn inhibits the differentiation and proliferation of Th2 and Th17 cells. IL-27 is a recently identified member of the pro-inflammatory and immunoregulatory IL-6/IL-12 cytokine family. IL-27 functions by binding to its receptors, WSX-1 and gp130, leading to the activation of the Jak/STAT signaling cascade, indicating that IL-27 is involved in the regulation of immune processes. Recombinant Mouse IL-27 is a non-disulfide-linked heterodimeric protein consisting of a 210-amino acid EBI3 subunit and a 206-amino acid IL-27-p28 subunit.
This recombinant product is provided as a lyophilized powder, featuring high activity, high purity, and low endotoxin.
Product Information
|
Product Name |
Product Number |
Specification |
|
Recombinant Mouse IL-27 Protein,His Tag |
90371ES10 |
10 μg |
|
90371ES50 |
50 μg |
|
|
90371ES80 |
1 mg |
Performance Parameters
|
Synonyms |
IL-27, h-IL-27, rh-IL-27, IL-27, interleukin-27 |
|
Species |
Mouse |
|
Source |
CHO Cells |
|
Sequence |
Y19-P228&F29-S234 |
|
Accession |
O35228&Q8K3I6 |
|
Tag |
No tag |
|
Molecular Weight |
Predicted molecular mass:48.8 kDa |
|
Endotoxin |
≤10 EU/mg by the LAL method |
|
Purity |
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining). |
|
Activity |
Measured in a VSV inhibition assay with Wish cells. The ED50 is ≤1 ng/mL. |
|
Formulation |
PBS, 5% Mannitol and 0.01% Tween 80, pH7.4. |
|
Appearance |
Lyophilized Powder |
Storage
Store at -25~-15°C. Valid for 1 year from the date of receipt. After reconstitution, store at 2~8°C for 7-10 days. After reconstitution, store at -85~-65°C for up to 6 months.
Reconstitution Method
Reconstitute with sterile deionized water to a concentration of 0.1-1 mg/mL. It is recommended to aliquot the protein upon first use to avoid repeated freeze-thaw cycles.
Notes
1. For your safety and health, please wear a lab coat and disposable gloves during operation.
2. This product is for research use only.
Documents:
Manuals
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.
You may also like
Inquiry
FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.