Description
IL-23 is a proinflammatory, heterodimeric protein composed of two subunits: a unique p19 subunit, and a p40 subunit that is shared with IL-12. IL-23 is secreted by activated dendritic cells and macrophages, and signals though a receptor comprised of IL-23R complexed with IL-12Rβ1. IL-23 has been shown to enhance proliferation of memory T cells.
Product Properties
|
Synonyms |
Interleukin-23 |
|
Source |
HEK293 Cells |
|
Molecular Weight |
Apparent molecular mass of 65 kDa, 43-45 kDa and 20 kDa in SDS-PAGE under reducing conditions. |
|
AA Sequence |
mouse IL23A (Q9EQ14) (Met1-Ala196 ) with a His tag at the C-terminus,& mouse IL12B (P43432) (Met1-Ser335) with a His tag at the C-terminus |
|
Biological Activity |
Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 0.5-4 ng/mL. |
|
Purity |
> 95% by SDS-PAGE. |
|
Endotoxin |
<1.0 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from sterile PBS, pH7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
|
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The product should be stored at -25~-15℃ for 1 year from date of receipt.
1 month, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.