Description
Interleukin-10 (IL-10) is an immunosuppressive cytokine produced by a variety of mammalian cell types, including macrophages, monocytes, T cells, B cells, and keratinocytes. IL-10 inhibits the expression of pro-inflammatory cytokines such as IL-1 and TNF-α. Similar to IL-4, IL-10 enhances humoral immune responses while attenuating cell-mediated immune responses. Human IL-10 is active on murine cells, but murine IL-10 is not active on human cells.
This recombinant mouse IL-10 is supplied as a lyophilized powder. It features high activity, high purity, low endotoxin levels, and is tag-free.
Components
|
Components No. |
Name |
90149ES05 |
90149ES10 |
90149ES50 |
90149ES60 |
90149ES76 |
|
90149 |
Recombinant Mouse IL-10 Protein |
2 μg |
10 μg |
50 μg |
100 μg |
500 μg |
Specifications
|
Synonyms |
Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, IL10A, TGIF, CSIF |
|
Source |
E.coli-derived mouse IL-10 protein, Ser19-Ser178. |
|
Accession |
NP_034678.1 |
|
Molecular Weight |
Approximately 18.6 kDa. |
|
AA Sequence |
SPGQGTQSEN SCTHFPGNLP NMLRDLRDAF SRVKTFFQMK DQLDNLLLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENQDPDIKAH VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFNK LQEKGIYKAM SEFDIFINYI EAYMTMKIRN |
|
Tag |
No Tag |
|
Endotoxin |
< 1.0 EU/μg by the LAL method. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC analyses. |
|
Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 106 IU/mg. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
Storage
This product should be stored at -25~-15℃ for 1 year. Once reconstituted under sterile conditions, it can be stored at 2 ~ 8°C for 7 days, or at -85 to -65°C for 3 months.
Reconstitution Instructions
Centrifuge the vial briefly before opening to pellet the contents at the bottom. Reconstitute with deionized water or PBS to a final concentration of 0.1–1.0 mg/mL. Further dilution can be performed as needed for downstream applications. For long-term storage, it is recommended to add a carrier protein (e.g., 0.1% BSA) to the dilution buffer. Aliquot the solution into single-use portions and store at -80°C to avoid repeated freeze-thaw cycles.
Notes
1. This product is for research use only.
2. Please operate with lab coats and disposable gloves，for your safety.
Documents:
Safety Data Sheet
Manuals:
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
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