Description
Interleukin-2 (IL-2) is a potent immunomodulatory lymphocyte cytokine produced by T cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is essential for T cell proliferation and other immune responses. The IL-2 receptor is composed of three subunits (55 kDa IL-2Rα, 75 kDa IL-2Rβ, and the 64 kDa common gamma chain γc/IL-2Rγ), which exist on the cell surface as distinct preformed complexes.
This recombinant mouse IL-2 contains 149 amino acid residues and is supplied as a lyophilized powder. It features high activity, high purity, and low endotoxin levels.
Components
|
Components No. |
Name |
90142ES08 |
90142ES10 |
90142ES50 |
90142ES60 |
90142ES76 |
|
90142 |
Recombinant Mouse IL-2 Protein |
2 μg |
10 μg |
50 μg |
100 μg |
500 μg |
Specifications
|
Synonyms |
Interleukin-2,IL-2,T-cell Growth Factor, TCGF, Aldesleukin; |
|
Expression Region & Expression System |
Mouse IL-2 Protein is expressed from E.coli with no tag .It contains Ala21-Gln169. |
|
Accession |
|
|
Molecular Weight |
Approximately 17.2 kDa |
|
AA Sequence |
APTSSSTSSS TAEAQQQQQQ QQQQQQHLEQ LLMDLQELLS RMENYRNLKL PRMLTFKFYL PKQATELKDL QCLEDELGPL RHVLDLTQSK SFQLEDAENF IS NIRVTVVK LKGSDNTFEC QFDDESATVV DFLRRWIAFC QSIISTSPQ |
|
Endotoxin |
< 1.0 EU/μg by the LAL method. |
|
Purity |
> 95%(determined by SDS-PAGE and HPLC) |
|
Biological Activity |
The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.2 ng/mL, corresponding to a specific activity of > 5.0 × 106 IU/mg. Fully biologically active when compared to standard. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
|
Physical Appearance |
White lyophilized powder |
Storage
This product should be stored at -25~-15℃ for 1 year. Once reconstituted under sterile conditions, it can be stored at 2 ~ 8°C for 7 days, or at -85 to -65°C for 3 months.
Reconstitution Instructions
Centrifuge the vial briefly before opening to pellet the contents at the bottom. Reconstitute with sterile deionized water to a final concentration of 0.1–1.0 mg/mL. Further dilution can be performed as needed for downstream applications. For long-term storage, it is recommended to add a carrier protein (e.g., 0.1% BSA) to the dilution buffer. Aliquot the solution into single-use portions and store at -80°C to avoid repeated freeze-thaw cycles.
Notes
1. This product is for research use only.
2. Please operate with lab coats and disposable gloves，for your safety.
Documents:
Safety Data Sheet
Manuals:
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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