Description
The MycAway™ Plus-Color One-Step Mycoplasma Detection Kit is a rapid detection product for mycoplasma contamination in cell culture which was developed based on the unique isothermal amplification technology of YEASEN. Compared with the previous mycoplasma detection products, this kit is optimized and has greatly reduced the false positive rate, improved the accuracy of the test, and enhanced the identification of negative and positive results. At the end of amplification, the sample can be left at room temperature for a period of time without the change from negative to positive, and thus will not affect the judgment of the results.
The principle is that if the cell culture is contaminated with mycoplasma, the conserved sequences of mycoplasma DNA will be amplified heavily and rapidly, causing the color of the reaction solution to change from blue-purple to sky-blue, and the result is visible to the naked eye, without electrophoresis.
MycAway™ Plus-Color One-Step Mycoplasma Detection Kit can detect multiple mycoplasma species, including eight that are common in cell culture. The traditional nested PCR method for mycoplasma detection is susceptible to influence by the inhibitors in cell culture supernatant and showed a false-negative result. Also if needed to perform the electrophoresis, cap opening may increase the contamination and lead to a false-positive result. The one-step mycoplasma detection kit has none of these disadvantages, also its detection sensitivity and accuracy are much higher than those of the PCR method.
Yeasen offers comprehensive solutions for Mycoplasma contamination. Explore our related products: Concept of Mycoplasma and the Impact of Contamination
Feature
- The one-step operation results in less than 60 minutes
- Use a water bath or PCR instrument to test
- 22 mycoplasma species were accurately detected by the kit, including 8 common species
- Results just identified by the visual inspection
Application
- Mycoplasma Detection
Components
| Components No. | Name | 40612ES25 （25 T) | 40612ES60 (100 T) |
| 40612-A | MycAway™ -Color Lamp | 425 μL | 4 × 425 μL |
| 40612-B | MycAway™-Color Lamp Primer | 50 μL | 4 × 50 μL |
| 40612-C | Positive Control | 10 μL | 4 × 10 μL |
| 40612-D | Mineral oil | 500 μL | 4 × 500 μL |
Shipping and Storage
All the components are shipped with dry ice and can be stored at -15℃ ~ -25℃ for 18 months. Please keep away from the light.
Figures
- The detection procedure
Figure 1. The detection procedure by MycAway™ Plus–Color One-Step Mycoplasma Detection Kit from YEASEN
FAQ
Q: What is the detection principle of the 40612 one-step rapid mycoplasma test kit (anti-contamination version)?
A: This kit contains UDG enzyme to remove the aerosol contamination of PCR product containing dU, which greatly inhibits false positive reactions and improves the accuracy of the test. Moreover, the added UDG enzyme functions at room temperature and is temperature-sensitive and prone to inactivation, without affecting the experimental results. If the cell culture is contaminated by mycoplasma, the conserved sequences of mycoplasma DNA will be amplified in large quantities and rapidly, causing the reaction solution to change from blue-purple to sky-blue. The result can be easily identified by the naked eye and no electrophoresis is required.
Q: How to use the one-step rapid mycoplasma detection kit (anti-contamination version)?
A: This reagent kit is much simpler to use. Just take 1 μL of the suspected mycoplasma-contaminated cell culture supernatant and add it to the reaction system. First, place the sample at 25-37 ℃ for 5 minutes, then incubate it at a constant temperature of 63 °C in a water bath or PCR instrument for 60 minutes. Observe that the reaction solution changes from blue-purple to sky-blue, and the result can be clearly seen without the need for electrophoresis.
Q: Using the 40612 one-step mycoplasma detection kit, we tested for the presence of mycoplasma contamination in organic solvents such as DMSO and ethanol. We found that the experimental group showed a sky-blue color. Does this mean that there is mycoplasma contamination in these organic solvents?
A: The mycoplasma kit is unstable and prone to degradation in ethanol, DMSO organic solvents, and in environments with strong acids and strong bases. As a result, the color changes to a sky blue. This kit can only be used for cell detection and should not be used in organic solvents or in environments with strong acids and strong bases for detection.
Q: I tested the liquid inside this. After adding it, the color in the PCR tube immediately turned sky blue. What's the reason for this? When testing pancreatic enzymes - EDTA, the color changed directly after adding the sample?
A: 40612 Principle: Mg2++HNB = Blue color. As PCR increases, pyrophosphate accumulates continuously. Pyrophosphate combines with Mg2+, while HNB remains free, resulting in a sky blue color. This reagent contains sodium dihydrogen phosphate, which may be the reason for the immediate chemical reaction between this ion and Mg2+ causing the reaction to occur and change color. EDTA can chelate calcium and magnesium ions, thereby accelerating the color change reaction.
Q: What is the concentration of the positive control?
A: 10 to the power of 5 copies per ul.
Q: Is it a mycoplasma in the positive control?
A: No, it's a linearized segment.
Documents
Citations & References:
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