Description
Insulin is a double-chain (α, β) polypeptide hormone secreted by islet β cells in the pancreas. It consists of 51 amino acids, of which the α chain contains 21 amino acids and the β chain contains 30 amino acids. The two chains are connected by two pairs of disulfide bonds, and the α chain itself also contains a pair of disulfide bonds. The secretion process of insulin is: the amino terminus of preproinsulin carries a 23-30 amino acid signal peptide. This signal peptide can transfer preproinsulin to the endoplasmic reticulum, and then is immediately cut off to release proinsulin. Proinsulin is composed of insulin β chain, C-peptide and α chain. The released proinsulin is immediately transferred to the Golgi apparatus, where it is packaged and converted into insulin, and is released into the blood together with C-peptide. Insulin is the only protein hormone known in the body that can lower blood sugar. It is not only responsible for regulating the uptake, utilization and storage of glucose, amino acids and fatty acids by cells, but also inhibits the degradation of glycogen, protein and fat.
This product is a full-length sequence of human insulin expressed in E. coli. The molecular weight is approximately 5800 Da. The structure is the same as that of naturally derived human insulin. It is commonly used as an auxiliary component in cell culture. The common concentration is 1~10 μg/mL.
Product Information
|
Cat NO. |
40112ES25 / 40112ES60 / 40112ES76 / 40112ES80 |
|
Specification |
25 mg / 100 mg / 500 mg / 1 g |
|
Source |
E.coli. |
|
CAS Number |
11061-68-0 |
|
Molecular Formula |
C257H383N65O77S6 |
|
Molecular Weight |
5808 |
|
Appearance |
White powder |
|
Activity |
≥ 15 U/mg |
|
Endotoxin |
<10 EU/mg |
|
Host Cell Protein |
< 10 ng/mg |
|
Microbial Limit |
< 300 cfu/g |
|
Purity |
95%-105% |
|
Sequence |
Gly-Ile-Val-Glu-Gln-Cys-Cys-Thr-Ser-Ile-Cys-Ser-Leu-Tyr-Gln-Leu-Glu-Asn-Tyr-Cys-Asn)-(Phe-Val- Asn-Gln-His-Leu-Cys-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cys-Gly-Glu-Arg-Gly-Phe-Phe- Tyr-Thr-Pro-Lys-Thr) [Disulfide bonds Cys6/Cys11 in first branch, 7 -7 between branches, 20 -19 between branches |
Storage
The product should be stored at -25~-15℃ for 3 years in lyophilized state
6 months, -25~-15℃ under sterile conditions after reconstitution.
Instructions for Use
Recombinant human insulin is supplied in powder form. This product is usually dissolved in a dilute hydrochloric acid solution (10mM) to prepare a stock solution containing 1-2mg per 1ml, which is then sterilized and filtered. The filtrate can be stored at -20 ℃ for 6 months after packaging. Repeated freezing and thawing should be avoided.
Cautions
1. Avoid aeration and bubble formation during dissolution and filtration as they may cause protein denaturation.
2. Insulin solution should not be autoclaved.
3. For your safety and health, please wear a lab coat and disposable gloves during operation.
4. This product is for research use only.
Citations & References:
[1] Chen S, Zhu G, Yang Y, et al. Single-cell analysis reveals transcriptomic remodellings in distinct cell types that contribute to human prostate cancer progression. Nat Cell Biol. 2021;23(1):87-98. doi:10.1038/s41556-020-00613-6(IF:28.824)
[2] Niu B, Liu J, Lv B, et al. Interplay between transforming growth factor-β and Nur77 in dual regulations of inhibitor of differentiation 1 for colonic tumorigenesis. Nat Commun. 2021;12(1):2809. Published 2021 May 14. doi:10.1038/s41467-021-23048-5(IF:14.919)
[3] Li S, Han S, Zhang Q, et al. FUNDC2 promotes liver tumorigenesis by inhibiting MFN1-mediated mitochondrial fusion. Nat Commun. 2022;13(1):3486. Published 2022 Jun 17. doi:10.1038/s41467-022-31187-6(IF:14.919)
[4] Li SL, Yang QQ, Liu XY, et al. Zn-doped Cu2S quantum dots as new high-efficiency inhibitors against human insulin fibrillation based on specific electrostatic interaction with oligomers. Int J Biol Macromol. 2021;179:161-169. doi:10.1016/j.ijbiomac.2021.03.012(IF:6.953)
[5] Usman M, Li Y, Ke Y, et al. Trappc9 Deficiency Impairs the Plasticity of Stem Cells. Int J Mol Sci. 2022;23(9):4900. Published 2022 Apr 28. doi:10.3390/ijms23094900(IF:5.924)
[6] Yang M, Zhang M, Liu Q, et al. 18β-Glycyrrhetinic acid acts through hepatocyte nuclear factor 4 alpha to modulate lipid and carbohydrate metabolism. Pharmacol Res. 2020;157:104840. doi:10.1016/j.phrs.2020.104840(IF:5.893)
[7] Wang BB, Wang YY, Zhang XY, et al. Bifunctional carbon dots for cell imaging and inhibition of human insulin fibrillation in the whole aggregation process. Int J Biol Macromol. 2020;147:453-462. doi:10.1016/j.ijbiomac.2019.12.267(IF:5.162)
[8] Yang QQ , Jin JC , Xu ZQ , et al. Active site-targeted carbon dots for the inhibition of human insulin fibrillation. J Mater Chem B. 2017;5(10):2010-2018. doi:10.1039/c6tb02823c(IF:4.543)
[9] Zhou Z, Liu F, Wang L, et al. Inflammation has synergistic effect with nicotine in periodontitis by up-regulating the expression of α7 nAChR via phosphorylated GSK-3β. J Cell Mol Med. 2020;24(4):2663-2676. doi:10.1111/jcmm.14986(IF:4.486)
[10] Bi Y, Huang W, Yuan L, et al. HOXA10 improves endometrial receptivity by upregulating E-cadherin†. Biol Reprod. 2022;106(5):992-999. doi:10.1093/biolre/ioac007(IF:4.285)
[11] Shi H, Li XY, Chen Y, et al. Quercetin Induces Apoptosis via Downregulation of Vascular Endothelial Growth Factor/Akt Signaling Pathway in Acute Myeloid Leukemia Cells [published correction appears in Front Pharmacol. 2021 Feb 08;11:640750]. Front Pharmacol. 2020;11:534171. Published 2020 Dec 10. doi:10.3389/fphar.2020.534171(IF:4.225)
[12] Zou Y, Zhao Y, Xiao Z, et al. Comparison of Regenerative Effects of Transplanting Three-Dimensional Longitudinal Scaffold Loaded-Human Mesenchymal Stem Cells and Human Neural Stem Cells on Spinal Cord Completely Transected Rats. ACS Biomater Sci Eng. 2020;6(3):1671-1680. doi:10.1021/acsbiomaterials.9b01790(IF:4.152)
[13] Wang J, Han L, Chen J, Xie Y, Jiang H, Zhu J. Reduction of non-specific toxicity of immunotoxin by intein mediated reconstitution on target cells. Int Immunopharmacol. 2019;66:288-295. doi:10.1016/j.intimp.2018.11.039(IF:3.118)
[14] Chen JX, Li HY, Li TT, et al. Alisol A-24-acetate promotes glucose uptake via activation of AMPK in C2C12 myotubes. BMC Complement Med Ther. 2020;20(1):22. Published 2020 Jan 29. doi:10.1186/s12906-019-2802-3(IF:2.838)
[15] Li D, Wei X, Ma M, et al. FFJ-3 inhibits PKM2 protein expression via the PI3K/Akt signaling pathway and activates the mitochondrial apoptosis signaling pathway in human cancer cells. Oncol Lett. 2017;13(4):2607-2614. doi:10.3892/ol.2017.5761(IF:1.390)
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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