Anti-Flag affinity purification gel (secretory protein or Membrane Protein) is prepared by covalent coupling of high-quality mouse IgG2b monoclonal antibody (clone number: 3F5) and 4% highly cross-linked agarose gel. This product has a high loading capacity of Flag-tagged fusion protein (at least 1.1 mg protein/mL gel) and less non-specific binding of impurities. It can be used for purification and immunoprecipitation (IP) of fusion proteins (secretory protein or membrane protein) with Flag tags.

Anti-Flag affinity purification gel (secretory protein or Membrane Protein) can bind to Met-modified N-terminal Flag fusion protein (Met-FLAG–Protein), N-terminal Flag fusion protein (FLAG–Protein), and C-terminal Flag fusion protein (Protein-FLAG).

Features

• High specificity: High-quality mouse IgG2b monoclonal antibody is used, which can specifically adsorb the Flag protein sequence.
• High loading capacity: Loading capacity ≥ 1.1 mg protein/mL gel.
• High purity: Less non-specific binding of impurities, resulting in high-purity Flag-tagged protein.
• Wide range of uses: Can be used for protein purification or IP.
• Multiple types of binding proteins: Can bind to Met-modified N-terminal Flag fusion protein (Met-Flag–Protein), N-terminal Flag fusion protein (Flag–Protein), C-terminal Flag fusion protein (Protein-Flag)

Specifications

Shipping and Storage

This product should be stored at 2~8℃ for 1 year. Cryopreservation in solutions without glycerol is prohibited!

Application

Purification of secreted or membrane proteins, immunoprecipitation（IP

Figures

1. High specificity and efficiently

Figure 1. Exhibits high specificity and efficiently binds Flag tags at any position, even at very low target protein expression levels using Anti-DYKDDDDK (Flag) Affinity Gel

2. Improved target protein yield and purity

Figure 2. Anti-DYKDDDDK (Flag) Affinity Gel has higher yield and purity of secretory or membrane proteins compared to other suppliers.

Publication

1. Xiao J, Wang S, Chen L, et al. 25-Hydroxycholesterol regulates lysosome AMP kinase activation and metabolic reprogramming to educate immunosuppressive macrophages. Immunity. 2024;57(5):1087-1104.e7. doi:10.1016/j.immuni.2024.03.021(IF:32.4)
2. Zheng Z, Zeng X, Zhu Y, et al. CircPPAP2B controls metastasis of clear cell renal cell carcinoma via HNRNPC-dependent alternative splicing and targeting the miR-182-5p/CYP1B1 axis. Mol Cancer. 2024;23(1):4. Published 2024 Jan 6. doi:10.1186/s12943-023-01912-w(IF:37.3)

Documents:

Safety Data Sheet

20585_MSDS_HB250613_EN.PDF

Manuals

20585_Manual_Ver.EN20250613.pdf