Description
Flag polypeptide is a hydrophilic polypeptide encoding 8 amino acids (DYKDDDDK). It can be used to detect specific anti-Flag monoclonal antibodies. In order to improve the sensitivity of detection, 3×Flag repeated polypeptide (3× flag-tag Peptide) has been developed. This 3×Flag repeated polypeptide contains 23 amino acids and can detect fusion proteins up to 10 fmol. 3×Flag repeated peptides have been widely used to purify Flag fusion proteins and competitively bind Anti-Flag monoclonal antibodies.
The recommended working concentration of 3×Flag repeated polypeptide is 100-400 μg/mL.
Features
- High stability
- High purity
- Short delivery
Applications
- Purify Flag fusion proteins
- Competitively bind Anti-Flag monoclonal antibodies.
Specifications
Amino acid sequence | H-Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH |
Molecular Formula | C120H169N31O49S |
Molecular Weight | 2861.87 |
Appearance | White Powder |
Purity | ≥98% |
Solubility | Soluble in water (10mM) or 1% acetic acid |
Components
Components No. | Name | 20571ES11 | 20571ES25 |
20571 | 3×Flag-tag Peptide | 4 mg | 25 mg |
Storage
The unopened products should be stably stored at -25℃ ~ -15℃ for 1 year. Storage solution should store at -85℃ ~ -65℃.