Hifair™ Lyo-Ready I Multiplex One Step RT-qPCR Kit（UDG plus) is an optimized reagent for fast and sensitive amplification and quantitation of RNA targets with probe-based assays. In the process of the experiment, reverse transcription and quantitative PCR were carried out in the same tube, which simplified the experimental operation and reduced the risk of contamination.The unique Buffer and enzyme design ensures that it can be used in the preparation and product design of one-step RT-qPCR lyophilized reaction systems.

The kit mainly contains Hifair™ V Reverse Transcriptase, Hieff UniconTM HotStart Taq DNA Polymerase and optimized lyophilizable buffer,etc. In addition, the factors that can effectively inhibit the non-specific PCR amplification and improve the amplification efficiency of multiple qPCR reactions are added, which can ensure the amplification efficiency and carry out up to multiple amplification reaction.

This kit is fitted with lyoprotectant that can be used for lyophilization.

Features

• Broad Compatibility: Compatible with both direct-use and freeze-dried formats for long-term storage
• High Stability: Maintains excellent stability after lyophilization, even when stored at 50°C for up to 33 days
• Flexible Lyophilization Formats: Supports various formats including 8-tube strip in-situ lyophilization, vial-based lyophilization, and micro-core freeze-dried forms
• Rapid Workflow: Delivers results in as fast as 40 minutes with an optimized fast-cycling protocol
• Effective Contamination Control: Incorporates a UDG/dUTP system for efficient prevention and removal of carryover contamination

Application

Virus detection; pathogen detection; gene expression analysis;

Specifications

Components

【Note】: 1) 5×Hifair™ Lyo-Buffer is the abbreviation of Lyo-Ready I Multiplex One Step RT-qPCR kit.

2) Hifair™ Lyo-Enzyme Mix mainly contains heat-resistant Hifair™ V Reverse Transcriptase, UNICONTM HotStart Taq DNA Polymerase and UDGase.

Shipping and Storage

This product should be stored at -25~-15℃ for one years. It should avoid repeated freezing and thawing.

Figures

1. Freeze-dried products in various forms

Figure 1. Freeze-dried product form

In situ freeze-drying and freeze-dried microspheres.  The freeze-dried morphology was good after freeze-drying, there was no collapse on the in situ freeze-dried surface, and there was no gap in the tube; the freeze-dried microspheres were plump and smooth in shape and uniform in size.

2. Consistent performance

Figure 2. Consistent performance between Liquid reagent and lyophilized Microspheres

3. Stability test

 

Figure 3. Freeze-dried microspheres accelerate stability test

After being freeze-dried into freeze-dried microspheres using Yeasen's RNA lyophilizable reagent (Catt#16645ES),  placed under different conditions (accelerated at 50°C for 33 days), and 10⁴, and 10³ copies/mL of HPIV pseudovirus templates were amplified simultaneously. The results showed that there was no significant change in the Ct value, fluorescence value, spherical shape, etc. of the freeze-dried microspheres before and after thermal acceleration, and the freeze-dried product had good stability.

4. Mutiplex PCR Amplification of pathogens

 Figure 4. Multiplex amplification of pathogens

(HPIVs, Template concentration E6/5/4/3/2/1)

Documents:

Safety Data Sheet

16645_MSDS_HB250520_EN.PDF

Manuals

16645_Manual_Ver.EN20250520.pdf

Citations & References:

Related blog:

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