Bsu DNA Polymerase (Large Fragment, 50 U/μL) _ 14415ES
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Bsu DNA Polymerase (Large Fragment, 50 U/μL) _ 14415ES
Bsu DNA Polymerase (Large Fragment, 50 U/μL) _ 14415ES
Bsu DNA polymerase (Large Fragment) is derived from Bacillus subtilis. This enzyme retains the 5′-to-3′ polymerase activity of Bsu DNA polymerase I but lacks the 5′-to-3′ exonuclease domain. The large fragment itself lacks 3′-to-5′ exonuclease activity. It is a DNA isothermal amplification polymerase with strand-displacement activity and is mainly used in Recombinase Polymerase Amplification (RPA). Product Information Catalog No. 14415ES72 / 14415ES80 / 14415ES90 / 14415ES97 Size 2,500 U / 5,000 U / 50,000 U / 250,000 U Unit Definition One unit (U) is defined as the amount of enzyme...

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Bsu DNA Polymerase (Large Fragment, 50 U/μL) _ 14415ES

YeasenSKU: 14415ES72
Size: 2,500 U
Price:
$715.00
Stock:
In stock

Bsu DNA polymerase (Large Fragment) is derived from Bacillus subtilis. This enzyme retains the 5′-to-3′ polymerase activity of Bsu DNA polymerase I but lacks the 5′-to-3′ exonuclease domain. The large fragment itself lacks 3′-to-5′ exonuclease activity. It is a DNA isothermal amplification polymerase with strand-displacement activity and is mainly used in Recombinase Polymerase Amplification (RPA).

Product Information

Catalog No.

14415ES72 / 14415ES80 / 14415ES90 / 14415ES97

Size

2,500 U / 5,000 U / 50,000 U / 250,000 U

Unit Definition

One unit (U) is defined as the amount of enzyme required to incorporate 10 nmol of dNTPs into acid-insoluble material within 30 min at 37°C.

Storage Buffer

25 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25°C.

Heat Inactivation

75°C for 20 min.

Component Information

Product Name

14415ES72
(2,500 U)

14415ES80
(5,000 U)

14415ES90
(50,000 U)

14415ES97
(250,000 U)

Bsu DNA Polymerase (Large Fragment, 50 U/μL)

50 μL

100 μL

1 mL

5 × 1 mL

Feature

  • Due to the lack of 3′-to-5′ exonuclease activity, Bsu DNA polymerase (Large Fragment) cannot remove unpaired 3′ overhangs and is therefore not suitable for generating blunt ends.
  • At 25°C, Bsu DNA polymerase (Large Fragment) retains 50% activity, which is twice that of Klenow Fragment (3′-to-5′ exo-) at the same temperature.
  • Storage buffer: 25 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25°C.
  • Heat inactivation: 75°C for 20 min.

Storage

Store at -25°C to -15°C. The shelf life is 2 years.

Documents:

Safety Data Sheet

14415_MSDS_HB260819

Manuals:

14415_Manual_HB20260818

Storage

Store at -25°C to -15°C. The shelf life is 2 years.

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