Description
Exonuclease III (E. coli) is a 3′ -> 5′ exonuclease which acts by digesting one strand of a dsDNA duplex at a time or digesting the RNA strand of an RNA-DNA heteroduplex. Exonuclease III (E. coli) breaks phosphodiester bonds on the 5′-side of AP sites in both dsDNA and ssDNA. It removes 3′-terminal groups on dsDNA, increases MutY turnover, and efficiently degrades 3′-recessed but not 3′ protruding DNA ends (creating 5′-overhangs). Exonuclease III (E. coli) removes a limited number of nucleotides per binding event, resulting in coordinated progressive deletions within the population of DNA molecules.
Applications
Degrades excess single-stranded primer oligonucleotides from a reaction mixture containing double-stranded extension products
Components
|
Name |
14525ES90 5 KU |
14525ES96 25 KU |
|
|
14525-A |
Exonuclease III (10 U/µL) |
50 μL |
5x 50 μL |
|
14525-B |
10 x Exo III Buffer |
500 μL |
5x 500 μL |
Shipping and Storage
The products should be stored at -25℃ ~ -15℃ for 2 years.
Documents:
Safety Data Sheet
Manuals
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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