Hieff Unicon™ Lyo-Ready I TaqMan Multiplex qPCR Mix -11893ES

SKU: 11893ES60

Size: 100 T
Price:
Sale price$135.00

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Description

Hieff Unicon™ Lyo-Ready I TaqMan Multiplex qPCR Kit is a fluorescence quantitative qPCR lyophilization reagent using the heat start Taq enzyme using a new generation antibody method, Suitable for FAM / HEX / TET / JOE / ROX double probe (this product is also suitable for Molecular Beacon probe), Reagents contain components including lyophilized protectants, heat start Fast Taq DNA polymerase, dNTP, Can be directly lyophilized on the machine. This product does not contain ROX correction dye, suitable to various Quantitative PCR models.

Specifications

Hot Start

Built-in hot start

Detection method

Primer-probe detection

PCR method

qPCR

Polymerase

Taq DNA polymerase

Type of sample

DNA

Application equipment

Applied Biosystems: 5700, 7000, 7300, 7700, 7900HT Fast, StepOne™, StepOne Plus™, 7500, 7500 Fast, ViiA™7, QuantStudio™ 3 and 5, QuantStudio™ 6,7,12k Flex;

Bio-Rad: CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4;

Eppendorf: Mastercycler ep realplex, realplex 2 s;

Qiagen: Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000;

Roche Applied Science: LightCycler 480, LightCycler 2.0; Lightcycler 96;

Stratagene: MX3000P™, MX3005P™, MX4000P™;

Thermo Scientific: PikoReal Cycler; Cepheid: SmartCycler; Illumina: Eco qPCR;

SLAN:SLAN-96S,SLAN-96P..  

Components

Components No.

Name

11893ES60 

11893ES80

11893

Lyo-ReadyTaqMan qPCR Mix

100 T

1,000 T

Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for 1 year. .

Instructions

  1. Lyophilized and machine loading system

Components

Volume (μL)

Final Concentration

Lyo-ReadyTaqMan qPCR Mix

20

Primer mix (10 μmol/L)

x

0.1-0.5 μmol/L

Probe mix (10 μmol/L)

x

50-250 nmol/L

Total Volume

21-25

-

*The primer concentration can be adjusted appropriately according to the experimental requirements, and the total volume should not exceed 5 μL.

  1. Lyophilization procedure

Step

Temperature

Set time

Duration

Vacuum degree

Pre-Freezing

-45℃

10 min

60 min

——

One-time dry stage

-45 ℃

10min

60min

0.01mbar

-40 ℃

10min

600min

0.01mbar

-35 ℃

10min

120min

0.01mbar

-30 ℃

20min

60min

0.01mbar

-20 ℃

20min

60min

0.01mbar

-10 ℃

20min

60min

0.01mbar

0 ℃

20min

60min

0.01mbar

Pressure Rise Test

Analyze the drying stage

5 ℃

10min

20min

0.01mbar

10 ℃

10min

20min

0.01mbar

15 ℃

10min

20min

0.01mbar

20 ℃

10min

20min

0.01mbar

25 ℃

10min

240min

0.01mbar

Pressure Rise Test

  1. Reaction System

Components

Volume (μL)

Final Concentration

20 μL Lyo-Bead

1 bead

/

Primer mix (10 μmol/L)

x

0.1-0.5 μmol/L

Probe mix (10 μmol/L)

x

50-250 nmol/L

Template

1-25

ddH2O

up to 25

-

*The template is dissolved in ddH2O, and the elution solution of the nucleic acid extraction reagent is using ddH2O.

  1. Reaction program

Cycle step

Temp.

Time

Cycles

Initial denaturation

95℃

5 min

1

Denaturation

95℃

15 s

45

Annealing/Extension

60℃

30 s

*The annealing / extension temperature can be adjusted appropriately according to the experimental requirements.

 Notes

Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety

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