Description
Hieff Clone™ Zero TOPO-TA/Blunt Cloning Kit is developed based on the principle of a next-generation topoisomerase that enables rapid and efficient ligation of DNA fragments. Coupled with a highly efficient blunt-ending factor, this kit supports fast cloning of both blunt-ended and sticky-ended PCR products.
Features
- Fast: Ligation completes in just 5 minutes.
- Highly Efficient: Virtually no self-ligation; near 100% positive clone rate—no blue-white screening needed.
- Broad Insert Range: Supports fragments from 150 bp to 5 kb.
- Simple Workflow: Just add your purified insert—no extra steps required.
- Versatile Compatibility: Enhanced with blunt-end binding factor for seamless TA cloning and blunt-end cloning.
Components
|
Components No. |
Name |
10906ES08 (5T) |
10906ES20 (20 T) |
10906ES50 (50 T) |
|
10906-A |
2× Hieff Clone™ Universal TOPO Cloning Premix |
25 μL |
100 μL |
250 μL |
|
10906-B |
1 kb control insert (40 ng/μL) |
10 μL |
10 μL |
10 μL |
|
10906-C |
M13 Forward Primer(10 μM) |
50 μL |
200 μL |
500 μL |
|
10906-D |
M13 Reverse Primer(10 μM) |
50 μL |
200 μL |
500 μL |
Storage
This product should be stored at room temperature for 1 year.
Application
Molecular Cloning; Topo Cloning; Rapid Cloning
Figures
1. Versatile Compatibility: TA cloning and blunt-end cloning.
Figure 1. High cloning efficiency of the Hieff Clone™ Universal Zero TOPO TA/Blunt Cloning Kit.
With 10 ng of a 1 kb insert (either blunt- or sticky-ended), the kit yields abundant colonies and achieves 100% positive clone rate.
2. Stong Stability
(A).Accelerated Stability
(B).Freeze-Thaw Stability
Figure 2. Stability Assessment
(A).Accelerated Stability: The Hieff Clone® Universal Zero TOPO TA/Blunt Cloning Kit remains stable after 7 days at 37°C.
(B).Freeze-Thaw Stability: The kit maintains performance after 50 freeze-thaw cycles.
Documents:
Safety Data Sheet
Manuals
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Inquiry
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FAQ
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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