Beschrijving
Hieff NGSTM MaxUp Human/Mouse/Rat rRNA Depletion Kit (rRNA & ITS/ETS) utilizes an RNase H digestion method to remove ribosomal RNA (rRNA) and the 45S & ITS/ETS regions from total RNA isolated from human, mouse, and rat samples, thereby enriching messenger RNA (mRNA) and other non-coding RNAs. This kit effectively removes rRNA from both intact and partially degraded total RNA (such as FFPE-derived RNA). Since degraded FFPE samples typically contain a higher proportion of ITS/ETS fragments compared to fresh tissue samples, the inclusion of specific probes targeting the human, mouse, and rat 45S & ITS/ETS regions in this kit significantly increases the proportion of usable sequencing data after depletion.
Features
- Strong Specificity: Efficiently removes rRNA as well as ITS/ETS regions from human samples, with excellent performance on challenging FFPE samples.
- Broad Input Compatibility: Suitable for a wide range of input amounts (100 ng to 1 μg).
- High Removal Efficiency: Achieves >95% removal of rRNA, ITS, and ETS from human, mouse, and rat samples.
- Consistent Quality: Strict batch-to-batch performance and stability controls ensure reliable results.
Components
|
Components No. |
Name |
12257ES12 |
12257ES24 |
12257ES96 |
|
12257-A |
Hybridization Buffer |
36 μL |
72 μL |
288 μL |
|
12257-B |
Human Probe Mix (rRNA & ITS/ETS) |
24 μL |
48 μL |
192 μL |
|
12257-C |
RNase H Buffer |
36 μL |
72 μL |
288 μL |
|
12257-D |
RNase H |
4 μL |
48 μL |
192 μL |
|
12257-E |
DNase I Buffer |
330 μL |
660 μL |
2x1320 μL |
|
12257-F |
DNase l |
30 μL |
60 μL |
240 μL |
Applications
- Gene Expression Research
- Alternative splicing analysis
- Detection and discovery of LncRNA(Non coding RNA)
- dentification of selective polyadenylation sites
- Fusion gene detection
Storage
This product should be stored at -25~-15℃ for 12 months.
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FAQ
Het product is alleen bedoeld voor onderzoeksdoeleinden en is niet bedoeld voor therapeutisch of diagnostisch gebruik bij mensen of dieren. Producten en inhoud worden beschermd door patenten, handelsmerken en auteursrechten die eigendom zijn van
Voor bepaalde toepassingen zijn mogelijk aanvullende intellectuele eigendomsrechten van derden vereist.