Beschrijving
Product Properties
|
Alternative Names |
GDF-15, MIC-1, NAG-1, PDF, PLAB, PTGFB, GDF15, MIC1, RG-1, Placental TGF-beta, PTGF-beta, PTGFBPTGF-beta, Placental TGF-β, PTGF-β, PTGFBPTGF-β |
|
Species |
Canine |
|
Expression Sequence |
Ala197-Val307 |
|
Accession |
A0A8C0TNP6 |
|
Tag |
N-His |
|
Expression System |
E.coli |
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Molecular Weight |
The protein has a predicted MW of 13.12 kDa. The protein migrates to 15-20 kDa under reduced (R) condition and 23-26 kDa under Non reducing (N) condition based on SDS-PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE. |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
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Formulation |
Lyophilized from 0.22μm filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization. |
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Appearance |
Lyophilized Powder |
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Biological Activity |
【ELISA Data】 Immobilized Canine GDF15, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GDF15 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA . 【ELISA Data】 Immobilized Canine GDF15, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 48.3ng/ml determined by ELISA. |
|
Reconstitution Method |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9). |
Features
High purity, high activity, low endotoxin, high batch-to-batch consistency
Storage
Store at -25 to -15°C. The shelf life is 1 year from the date of receipt. After reconstitution, store at -85 to -65°C under sterile conditions for up to 3 months.
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