Beschrijving
Product Properties
|
Alternative Names |
CD47 glycoprotein, CD47 molecule, CD47, IAP, OA3, MER6 |
|
Species |
Rat |
|
Expression Sequence |
Gln19-Lys140 |
|
Accession |
P97829-1 |
|
Tag |
C-hFc |
|
Expression System |
HEK293 |
|
Molecular Weight |
The protein has a predicted MW of 40.40 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on SDS-PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
|
Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Biological Activity |
【ELISA Data】 Immobilized Mouse SIRP alpha, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Rat CD47, hFc Tag with the EC50 of 29.8ng/ml determined by ELISA. |
|
Reconstitution Method |
Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. |
Features
High purity, high activity, low endotoxin, high batch-to-batch consistency
Storage
Store at -25 to -15°C. The shelf life is 1 year from the date of receipt. After reconstitution, store at -85 to -65°C under sterile conditions for up to 3 months.
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