Beschrijving
Product Properties
|
Alternative Names |
SCFR, PBT, CD117, ckit, c-kit, KIT |
|
Species |
Mouse |
|
Expression Sequence |
Ser25-Pro523 |
|
Accession |
P05532-2 |
|
Tag |
C-His |
|
Expression System |
HEK293 |
|
Molecular Weight |
The protein has a predicted MW of 56.63 kDa. Due to glycosylation, the protein migrates to 75-100 kDa based on SDS-PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Biological Activity |
【SPR Data】Mouse CD117, His Tag captured on CM5 Chip via anti-his antibody can bind Human SCF, No Tag with an affinity constant of 14.02 μM as determined in SPR assay . |
|
Reconstitution Method |
Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. |
Features
High purity, high activity, low endotoxin, high batch-to-batch consistency
Storage
Store at -25 to -15°C. Once received, the product is valid for one year. After reconstitution, store under sterile conditions at -85 to -65°C. The product is valid for three months.
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FAQ
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