Beschrijving
Product Properties
|
Alternative Names |
HLA G antigen, sHLA-G, b2 microglobulin, HLA G, HLAG, HLA-G, MHC-G, sHLA-G |
|
Species |
Cynomolgus |
|
Expression Sequence |
Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide |
|
Accession |
E0WKX9(HLA-G)&Q8SPW0(B2M)&RIIPRHLQL |
|
Tag |
C-His-Avi |
|
Expression System |
HEK293 |
|
Molecular Weight |
The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on SDS-PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Biological Activity |
【SPR Data】Cynomolgus LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Cynomolgus HLA-G&B2M&Peptide (RIIPRHLQL) Monomer, His Tag with an affinity constant of 0.13 μM as determined in SPR assay . |
|
Reconstitution Method |
Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. |
Features
High purity, high activity, low endotoxin, high batch-to-batch consistency
Storage
Store at -25 to -15°C. Once received, the product is valid for one year. After reconstitution, store under sterile conditions at -85 to -65°C. The product is valid for three months.
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