Beschrijving
Product Properties
|
Alternative Names |
Plasma kallikrein, PKK, KLK3, KLKB1, Fletcher factor, Kininogenin |
|
Species |
Cynomolgus |
|
Expression Sequence |
Gly20-Ala638 |
|
Accession |
A0A2K5VTJ9 |
|
Tag |
C-His |
|
Expression System |
HEK293 |
|
Molecular Weight |
The protein has a predicted MW of 70.19 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on SDS-PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in 20mM NaAc, 150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Biological Activity |
【ELISA Data】Immobilized Anti-KLKB1 Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus KLKB1, His Tag with the EC50 of 1.1μg/ml determined by ELISA. |
|
Reconstitution Method |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.0). |
Features
High purity, high activity, low endotoxin, high batch-to-batch consistency
Storage
Store at -25 to -15°C. Once received, the product is valid for one year. After reconstitution, store under sterile conditions at -85 to -65°C. The product is valid for three months.
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