Beschrijving
CD23 is a type 2 transmembrane C-type lectin that binds IgE, CD21, CD11b and CD11c. It is expressed on a variety of hematopoietic cells and functions in allergic immune responses. Recombinant human sCD23 is a 19.2 kDa non-glycosylated protein containing 172 amino-acid residues.
Product Properties
|
Alternative Names |
FCER2 |
|
Species |
Human |
|
Expression Sequence |
Met1-Ser321 |
|
Accession |
P06734 |
|
Tag |
No Tag |
|
Expression System |
E.coli |
|
Molecular Weight |
Approximately 19.2 kDa. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
< 0.1 EU per µg by the LAL method. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
|
Appearance |
Lyophilized Powder |
|
Biological Activity |
The ED50determined by its ability to induce TNF-alpha production by human PBMCs. Fully biologically active when compared to standard. |
|
Reconstitution Method |
Before opening the lid, centrifuge to bring the contents to the bottom. Reconstitute with sterile distilled water or PBS and reconstitute to a concentration of 0.1-1.0 mg/mL. It can be further diluted according to subsequent experiments. For long-term storage, it is recommended to add carrier protein (0.1% BSA) to the dilution buffer. Aliquot into the amount for one experiment and freeze at -80℃ to avoid repeated freezing and thawing. |
Features
High purity, high activity, low endotoxin, high batch-to-batch consistency
Storage
Store at -25 ~ -15℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months.
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FAQ
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