Beschrijving
Product Properties
|
Alternative Names |
IL-23-A,IL23p19,IL-23A,IL-23 P19 |
|
Species |
Human |
|
Expression Sequence |
Arg 20 - Pro 189 with hIgG1 Fc Tag at the C-Terminus |
|
Accession |
Q9NPF7 |
|
Tag |
Human IgG1 Fc |
|
Expression System |
HEK293 |
|
Molecular Weight |
The protein has a predicted MW of 45.43 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on SDS-PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE. |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
|
Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Activity |
Immobilized IL-23A(20-189) Fc Chimera Protein, Human at 1.0μg/mL (100μL/well) can bind Biotinylated Guselkumab with EC50 of 10.77-14.25 ug/mL. |
|
Reconstitution Method |
Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. Aliquot after initial reconstitution to avoid repeated freeze-thaw cycles. |
Features
High purity, high activity, low endotoxin, high batch-to-batch consistency
Figure
SDS- PAGE
Loading： 2μg (R: reducing , N: non-reducing).
ELISA
Immobilized IL-23A(20-189) Fc Chimera Protein, Human at 1.0μg/mL (100μL/well) can bind Biotinylated Guselkumab with EC50 of 10.77-14.25 ug/mL.
Storage
Store at -20 ~ -80℃, valid for 1 year after receipt. After reconstitution, store at -85 ~ -65℃ under sterile conditions, valid for 3 months.
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