Beschrijving
Product Properties
|
Property Name |
Property Details |
|
Alternative Names |
CD47,OA3,CD47 molecule,CD47 glycoprotein |
|
Species |
Human |
|
Expression Sequence |
Gln19-Pro139 |
|
Accession |
Q08722-1 |
|
Tag |
No Tag |
|
Expression System |
HEK293 |
|
Molecular Weight |
The protein has a predicted MW of 13.72 kDa. Due to glycosylation, the protein migrates to 35-48 kDa based on SDS-PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
< 1.0 EU per μg by the LAL method. |
|
Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized Powder |
|
Biological Activity |
【ELISA Data】 Immobilized Human CD47, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 0.24μg/ml determined by ELISA. |
|
Reconstitution Method |
Before opening, centrifuge briefly. Reconstitute the lyophilized protein with distilled water to a concentration exceeding 100 μg/mL. For initial use, aliquot the protein to avoid repeated freeze-thaw cycles. |
Features
High purity, high activity, low endotoxin levels, and high batch-to-batch consistency
Storage
Store at -25 to -15°C; valid for 1 year upon receipt.After reconstitution, store under sterile conditions at -85 to -65°C; valid for 3 months.
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