Beschrijving
Product Properties
|
Synonyms |
ETGF; TGF-alpha; TGF type 1; TGFA |
|
Species |
Human |
|
Source |
HEK293 |
|
Sequence |
Val40-Ala89 |
|
Accession |
P01135-1 |
|
Tag |
N-hFc |
|
Molecular Weight |
The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result. |
|
Endotoxin |
Less than 1EU per μg by the LAL method. |
|
Purity |
> 95% as determined by Bis-Tris PAGE and HPLC |
|
Activity |
ELISA Data: Immobilized Human TGF-alpha, hFc Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TGF-alpha Antibody, hFc Tag with the EC50 of 6.0ng/ml determined by ELISA
SPR Data: Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human TGF-alpha, hFc Tag with an affinity constant of 0.31 μM as determined in SPR assay. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in 50mM Tris,100mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
lyophilized powder |
Storage
Store at -25°C to -15°C. The product is valid for 1 year upon receipt.
After reconstitution, store at -85°C to -65°C and use within 3 months.
Reconstitution
Centrifuge before opening the vial. It is recommended to dissolve the lyophilized protein in distilled water. The reconstitution concentration should exceed 100 μg/ml.
Note
1.Please operate with lab coats and disposable gloves, for your safety.
2.This product is for research use only.
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FAQ
Het product is alleen bedoeld voor onderzoeksdoeleinden en is niet bedoeld voor therapeutisch of diagnostisch gebruik bij mensen of dieren. Producten en inhoud worden beschermd door patenten, handelsmerken en auteursrechten die eigendom zijn van
Voor bepaalde toepassingen zijn mogelijk aanvullende intellectuele eigendomsrechten van derden vereist.